| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,669,465 | 0 | C | A | 58.8% | ‑0.5 / 13.2 | 17 | L240F (TTG→TTT) | yphD | ABC transporter permease |
| Reads supporting (aligned to +/- strand): ref base C (2/5); new base A (0/10); total (2/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.54e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.50e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGC > NZ_CP009273/2669376‑2669541 | gCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCcaac > 1:598567/1‑90 (MQ=255) cgccgACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGc < 2:1159410/90‑1 (MQ=255) cgccgACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGc < 1:975698/90‑1 (MQ=255) gACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGt > 2:137981/1‑90 (MQ=255) cgcccGCGGCGATGACGCAAAACCCCAGCCCGTTTGCGGCCCCTGCGTTCCCAGAACCGAGGCGCGCCCCCAAAAAAATGCCGGTCAccg < 2:986725/87‑1 (MQ=255) gcgATGCCGTCAACCCCCAGCCCGTTTGCGGCACCTGCGTTACAAAACCCGAGGCCCGCCCCCAAAAAAAGGCCGGCCACCGCCGCtaat < 2:157056/90‑1 (MQ=255) tCAACCCCCAGACCGTTTGGGCCCCCTGCGTTCCCAAAACCGGGGCGCGCCGCCAAAAAAAGGCCGGTCCCCGCCGCTAATAATCCCGaa < 1:876729/90‑1 (MQ=255) cccAGCCTGTTTGGGGCCCCTGCGTTCCAAAAACGGGGGCGCCCCCCAAAAAAAATGCGGGTCACCGCCGCTAATAATCCCGAAAGGGTa < 2:270920/89‑1 (MQ=255) cccAGCCCGTTTGCGGCCCCTGCGTTCCCAGAACCGAGGCGCGCCGCAAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTa < 2:636112/89‑1 (MQ=255) cccAGCCCGTTTGCGGCCCCTGCGTTACCAAACCCGGGGCGCGCCGCAAAAAAAAGGCCGGTCCCCGCCGCTAATAATCCCGAAAGGGTa < 1:137981/89‑1 (MQ=255) cccAGCCCGTTTGCGCCCCCTGCGTTCCCAAAACCGAGCCGCCCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTa < 2:14513/89‑1 (MQ=255) cccAGACCGTTTGCGCCCCCTGCTTTTCAAAACCCGGGGCGCGCCGCCAAAAAATTGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTa < 2:384854/89‑1 (MQ=255) cccAAACCGTTTGCGGCACCTGCTTTCCCAAACCCGGGGCGCGCCCCAAAAAAAATGCCGGTCCCCGCCGCTAATAATCCCGAAAGGGTa < 1:374314/89‑1 (MQ=255) gTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAg > 1:47705/1‑90 (MQ=255) ttACCAAACCCGAGGCGCGCCCCCAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCcgacga < 2:91675/90‑1 (MQ=255) cccGGGGCGCCCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGAt < 2:1081486/89‑1 (MQ=255) ggCGCGCCCCCAAAAAAATCCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGc < 2:742881/90‑1 (MQ=255) cgcgctcCGACACCAAAAGCCCCGTCCCCGCCGCTAATAATCCCGAAAGGGCAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGc < 1:133638/89‑1 (MQ=255) | GCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGC > NZ_CP009273/2669376‑2669541 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |