| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 170,552 | 0 | A | C | 54.5% | 19.9 / 27.0 | 23 | V273G (GTA→GGA) | hemL | glutamate‑1‑semialdehyde 2,1‑aminomutase |
| Reads supporting (aligned to +/- strand): ref base A (9/1); new base C (0/12); total (9/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.01e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.45e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCGGGTTACCGGAAAGCGTACCCGCCTGATAGACCGGACCCGTCGGGGCCAGCGCATCCATTACATCACGACGACCACCGAATGCGCCTACCGGCATTCCACCGCCGATGATTTTGCCGAGGCAGGTTAAATCTGGCACTACGCCGTAATAATCCTGTGCGCCAGCTAGCGCTA > NZ_CP009273/170463‑170636 | tCGGGTTACCGGAAAGCGTACCCGCCTGATAGACCGGACCCGTCGGGGCCAGCGCATCCATTACATCACGACGACCACCGAATGCGCCTa < 2:718538/90‑1 (MQ=255) gTTACCGGAAAGCGTACCCGCCTGATAGACCGGACCCGTCGGGGCCAGCGCATCCATTACATCACGACGACCACCGAATGCGCCTACCgg > 2:459275/1‑90 (MQ=255) aaaGCGTCCCCGCCTGATAGCCCGGCCCCGTGGGGGCCAGCGCATCCATTACATCACGACGACCACCGAATGCGCCTCCCGGCATTCCAc < 1:365871/90‑1 (MQ=255) aaaGCGTACCCGCCTGATAGACCGGACCCGTCGGGGCCAGCGCATCCATTACATCACGACGACCACCGAATGCGCCTACCGGCATTCCAc > 1:577209/1‑90 (MQ=255) aaaGCGTACCCGCCTGATAGACCGGACCCGTCGGGGCCAGCGCATCCATTACATCACGACGACCACCGAATGCGCCTACCGGCATTCCAc > 2:398713/1‑90 (MQ=255) gTACCCGCCTGATAGACCGGACCCGTCGGGGCCAGCGCATCCATTACATCACGACGACCACCGAATGCGCCTACCGGCATTCCAccgccg > 1:481291/1‑90 (MQ=255) aCCGGGCCGGTCGGGCCCCCCCCATCCGTTACATCCCGGCGACCCCGGAAGGCCCCTTACTCCTCTCCACCGCCGATGATTTTGCCGAgg < 2:76236/90‑1 (MQ=255) ccGGCCCCGTCGGGCCCAGCCCACCCATTACAACACGACGCCCACCGAATGCCCCTCCCGGCATTCCACCGCCGATGATTTTGCCGAGGc < 2:98108/90‑1 (MQ=255) cGGACCCGTCGGGGCCAGCGCATCCATTACATCACGACGACCACCGAATGCGCCTACCGGCATTCCACCGCCGATGATTTTGCCGAGGCa > 1:291547/1‑90 (MQ=255) ccGTGGGGCCCGGCGCATCCATTACATCCCGACGCCCACGGAATGCCCCTCCCGGCATCCCACCGCCGATGATTTTGCCGAGGCAGGTTa < 2:450723/90‑1 (MQ=255) gTCGGGGCCAGCGCATCCATTACATCACGACGACCACCGAATGCGCCTACCGGCATTCCACCGCCGATGATTTTGCCGAGGCAGGTTaaa > 1:570258/1‑90 (MQ=255) cAGCGCACCCATTACTACCCGCCGCCCCCGGAATGCCCCTCCCGGCATTCCACCGCCGATGATTTTGCCGAGGCAGGTTAAATCTGGCAc < 1:685958/90‑1 (MQ=255) gcgcATCCATTACATGACGACAACCACCGAATGCGCCTACCGGCATTCCACCGCGGATGAGTTTGCCGAGGCAGGTTAAATCTGGCACTa > 2:553978/1‑90 (MQ=255) gcgcATCCATTACATCACGACGACCACCGAATGCGCCTACCGGCATTCCACCGCCGATGATTTTGCCGAGGCAGGTTAAATCTGGCACTa > 1:787110/1‑90 (MQ=255) ccATCCTTTACATCACGACGCCCCCCGAATGCCCCTCCCGGCATCCCACCGCCGATGATTTTGCCGAGGCAGGTTAAATCTGGCACTACg < 1:522674/89‑1 (MQ=255) ctCCATTACATCACGACGCCCCCCGAATGCGCCTCCCGGCATTCCACCGCCGATGATTTTGCCGAGGCAGGTTAAATCTGGCACTACGcc < 2:418567/89‑1 (MQ=255) ccccATTACACCACGCCGCCCCCCGCAGGCCCCCCCCGGCATTCCACCGCCGATGATTTTGCCGAGGCAGGTTAAATCTGGCACTACGcc < 2:408823/88‑1 (MQ=255) acccATTACATCACGACGCCCCCCGAATGCCCCTCCCGGCATCCCACCGCCGATGATTTTGCCGAGGCAGGTTAAATCTGGCACTACGcc < 1:799967/88‑1 (MQ=255) aTCCTTTACATCACGACGACCACCGAATGCGCCTCCCGGCTTTCCCCCGCCGATGATTTTGCCGAGGCAGGTTAAATCTGGCACTACGcc < 2:291547/90‑1 (MQ=255) aTCCATTACATCACGACGACCCCCGAATGCCCCTCCCGGCATTCCACCGCCGATGATTTTGCCGAGGCAGGTTAAATCTGGCACTACGcc < 1:125683/90‑1 (MQ=255) aTCCATTACACCACGCCGACCACCGAATGCGCCTCCCGGCATTCCACCGCCGATGATTTTGCCGAGGCAGGTTAAATCTGGCACTACGcc < 1:12976/90‑1 (MQ=255) aTCCATTACACCACGACGACCACCGAATGCGCCTACCGGCTTTCCACCGCCGATGATTTTGCCGAGGCAGGTTAAATCTGGCACTACGcc < 1:830848/90‑1 (MQ=255) aTCAATTACATCACGCCGCCCCCCGAATGCGCCTCCCGCCATTCCACCGCCGATGATTTTGCCGAGGCAGGTTAAATCTGGCACTACGcc < 2:748892/90‑1 (MQ=255) cgcCTACCGGCATTCCACCGCCGATGATTTTGCCGAGGCAGGTTAAATCTGGCACTACGCCGTAATAATCCTGTGCGCCAGCTAGCGCTa > 1:678721/1‑90 (MQ=255) | TCGGGTTACCGGAAAGCGTACCCGCCTGATAGACCGGACCCGTCGGGGCCAGCGCATCCATTACATCACGACGACCACCGAATGCGCCTACCGGCATTCCACCGCCGATGATTTTGCCGAGGCAGGTTAAATCTGGCACTACGCCGTAATAATCCTGTGCGCCAGCTAGCGCTA > NZ_CP009273/170463‑170636 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |