| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 760,187 | 0 | G | C | 61.1% | 3.8 / 21.7 | 18 | P184P (CCG→CCC) | sucD | succinate‑‑CoA ligase subunit alpha |
| Reads supporting (aligned to +/- strand): ref base G (1/6); new base C (11/0); total (12/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.77e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.92e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTGACCTATGAAGCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGA‑CCCGATCCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAGACCGAAGCGATCGTGATGATCGGTGAGATCGGCGG > NZ_CP009273/760104‑760270 | cTGACCTATGAAGCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGAGCCCGACCCCggggct > 1:530524/1‑87 (MQ=255) gACCTATGAAGCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGA‑CCCGATCCCGGGCTCTaa < 1:243979/90‑1 (MQ=255) aTGAAGCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGGGA‑CCCCATCCCCGGGCCTTACtttt > 1:180146/1‑89 (MQ=255) gCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGGGA‑CCCCAGCCCCGGGTCTTACTTTTTCCAc > 1:828819/1‑90 (MQ=255) tAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGGGG‑CCCCATCCCCGGCTCTAACTTTTTCTACATtct > 2:330684/1‑90 (MQ=255) gACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGGGA‑ACCCCCCCCCGGCGCTTAATTTTTTGACATTCTCCAAAt > 2:48716/1‑90 (MQ=255) ccACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGA‑CCCCATCACCGGCTCTAACTTTTTTGACATTCTCGAAATGt > 1:442614/1‑90 (MQ=255) cGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGA‑CCCCATCCCCGGCTCTAAATTTTTCGACATTTTCGTAAAGtttg > 1:494051/1‑88 (MQ=255) tACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGA‑CCCCGTCCCCGGCGCTTACTTTTTTCACATTTTCGGAAAGTTTGaaaaa > 1:205483/1‑90 (MQ=255) ttCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGA‑CCCCCTCCCCGGCTCTAACTTTTTTGACATTTTCGAAAAGTTCGGAAAAAAACCg > 1:568130/1‑90 (MQ=255) ttCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGA‑CCCCCTCCCCGGCCCTAACTTTATTTACATTTTCCCAAAGTTTGGAAAAAAACCg > 1:565404/1‑90 (MQ=255) ggTCAGTCGACCTGTGTCGGTATCGGCGGGGA‑CCCCCTCCCCGGCCCTAACTTTTTTTACATTCTCGAAATGTTTGAAAAAAAACCGCAg > 2:534835/1‑90 (MQ=255) tCGACCTGTGTCGGTATCGGCGGTGA‑CCCGATCCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAGACCGaa < 2:565404/90‑1 (MQ=255) tCGACCTGTGTCGGTATCGGCGGTGA‑CCCGATCCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAGACCGaa < 2:494051/90‑1 (MQ=255) tATCGGCGGTGA‑CCCGATCCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAGACCGAAGCGATCGTgatgat < 1:473872/90‑1 (MQ=255) tATCGGCGGTGA‑CCCGATCCCCGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAGACCGAAGCGATCGTgatgat > 1:47138/1‑90 (MQ=255) a‑CCCGATCCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAGACCGAAGCGATCGTGATGATCGGTGAGATcg < 2:408646/90‑1 (MQ=255) a‑CCCGATCCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAGACCGAAGCGATCGTGATGATCGGTGAGATcg < 2:180146/90‑1 (MQ=255) gATCCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAGACCGAAGCGATCGTGATGATCGGTGAGATcggcgg > 1:200738/1‑90 (MQ=255) | CTGACCTATGAAGCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGA‑CCCGATCCCGGGCTCTAACTTTATCGACATTCTCGAAATGTTCGAAAAAGATCCGCAGACCGAAGCGATCGTGATGATCGGTGAGATCGGCGG > NZ_CP009273/760104‑760270 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |