Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	49,898	G→A	A26T (GCC→ACC)	folA →	type 3 dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	49,898	0	G	A	100.0%	101.9 / NA	33	A26T (GCC→ACC)	folA	type 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/21);  total (12/21)

AAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGT  >  NZ_CP009273/49815‑49982                                                                                    |                                                                                     aaaTCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGAtc                                                                                <  1:493310/90‑1 (MQ=255)         aTGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCggg                                                                        >  1:35901/1‑90 (MQ=255)             tCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTa                                                                    >  1:508341/1‑90 (MQ=255)                 tCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACg                                                                >  1:496037/1‑90 (MQ=255)                    gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCaa                                                             <  1:540996/90‑1 (MQ=255)                    gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCaa                                                             <  1:606535/90‑1 (MQ=255)                    gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCaa                                                             <  2:508015/90‑1 (MQ=255)                    gATTGCGGCGCTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCaa                                                             <  1:314091/90‑1 (MQ=255)                     aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAac                                                            <  1:169347/90‑1 (MQ=255)                     aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAac                                                            <  2:808390/90‑1 (MQ=255)                     aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAac                                                            >  2:563158/1‑90 (MQ=255)                     aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAac                                                            >  2:511255/1‑90 (MQ=255)                     aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAac                                                            <  2:471463/90‑1 (MQ=255)                     aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAac                                                            <  2:257546/90‑1 (MQ=255)                         cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCt                                                        <  2:87270/90‑1 (MQ=255)                          ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCtt                                                       >  1:392541/1‑90 (MQ=255)                                 gCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTtaaataaa                                                <  2:35901/90‑1 (MQ=255)                                  cGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAAc                                               <  1:123294/90‑1 (MQ=255)                                      aGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGt                                           <  1:511255/90‑1 (MQ=255)                                      aGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGt                                           <  1:563158/90‑1 (MQ=255)                                           gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGcc                                                                           <  1:713403/53‑1 (MQ=255)                                           gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGcc                                                                           >  2:713403/1‑53 (MQ=255)                                           gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGGTTAAACGCAACACCTTAAATAAACCCGTGATTa                                      >  2:679979/1‑90 (MQ=255)                                           gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGGTTAAACCCAACACCTTAAATAAACCCGTGATTa                                      >  2:753348/1‑90 (MQ=255)                                            cgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTAt                                     <  2:242098/90‑1 (MQ=255)                                              ttATCGGCATGGAAAACGCCATGCCGTGGACCCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg                                   <  2:291735/90‑1 (MQ=255)                                              ttATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg                                   >  1:778531/1‑90 (MQ=255)                                                  cGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccg                               >  1:619822/1‑90 (MQ=255)                                                      aTGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt                           <  2:778531/90‑1 (MQ=255)                                                      aTGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt                           <  2:173430/90‑1 (MQ=255)                                                              cGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCaa                                                             <  1:72125/48‑1 (MQ=39)                                                              cGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCaa                                                             >  2:72125/1‑48 (MQ=38)                                                              cGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGa                   <  2:732581/90‑1 (MQ=255)                                                                               tgcctaccGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGGCGTCCGt  >  2:642199/1‑90 (MQ=255)                                                                                    |                                                                                     AAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGT  >  NZ_CP009273/49815‑49982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑