| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 807,177 | 0 | T | G | 52.6% | 18.9 / 24.4 | 19 | V67G (GTG→GGG) | bioC | malonyl‑ACP O‑methyltransferase BioC |
| Reads supporting (aligned to +/- strand): ref base T (3/6); new base G (10/0); total (13/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.10e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.61e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAAT > NZ_CP009273/807091‑807263 | tCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGAc < 2:51867/90‑1 (MQ=255) aGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCt < 1:279572/90‑1 (MQ=255) gCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGGGACGGCCtt > 1:234541/1‑90 (MQ=255) cGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGGCACGCGCAGGGGACGGCCTTa > 1:207097/1‑90 (MQ=255) ggACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGGGACGGCCTTAGATCTCTCGCCGCCAATGCt > 1:240793/1‑90 (MQ=255) aCGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGGGACGGCCTTAGATCTCTCGCCGCCAATGCTTg > 1:82386/1‑90 (MQ=255) gtgGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCAc < 1:338702/90‑1 (MQ=255) ctggctggATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGc > 1:141194/1‑82 (MQ=255) ctggctggATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGc < 2:141194/82‑1 (MQ=255) tggctggATGAGCCGCCACTGGCGGGAACGGCACCCGCAGGGGGGGGCCTTTGATTTCTCCCCGCCCATGCTTTTTCAGGCACCCCCGaa > 2:769766/1‑90 (MQ=255) ggATGAGCCGCCACTGGCGGGAACGTCACCCGCCGGGGGCGGCCCTAGATCTCTCCCCGCCCAAGCTTGTTCTGGCACCCCAGAAAGagg > 2:572463/1‑88 (MQ=255) gccgccACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAg < 2:681390/90‑1 (MQ=255) gccgccACTGGCGGGAAAGTCACGCGCAGGGGACGGCCTTAGATCTCTCGCCCCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAg > 1:156541/1‑90 (MQ=255) ccACTGGCGGGGACGTCACGCGCAGGGGACGGCCCTAGATCTCTCCCCCCCCAAGCTTGTTTAGGCACGCCAGAAGGAAGCCGCAGACCa > 2:705529/1‑90 (MQ=255) gggAACGTCACGCGCAGGGGGCGGCCTTAGATCTCTCGCCGCCCATGCTTGTTTAAGCACGCCAGAAGGATGCCGCCGACCCTTATCTgg > 2:113396/1‑90 (MQ=255) gAAAGTCACGCGCAGGGGACGGCCTTAGATCTCTCCCCCCCCATGCTTGTTTAGGCCCGCCAGAAGGATGCCGCAGACCATTATCTGGCg > 2:726165/1‑90 (MQ=255) aGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCAc < 1:94088/45‑1 (MQ=255) aGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCAc > 2:94088/1‑45 (MQ=255) aGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAAt > 2:649611/1‑90 (MQ=255) | TCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAAT > NZ_CP009273/807091‑807263 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |