| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,141,214 | 0 | T | G | 66.7% | 5.0 / 11.4 | 15 | G20G (GGT→GGG) | yegD | molecular chaperone |
| Reads supporting (aligned to +/- strand): ref base T (0/5); new base G (10/0); total (10/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.27e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTTCTTCACCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGACGGTAAACCGCATTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCGAA > NZ_CP009273/2141128‑2141298 | cttcttCACCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGGCGGGaaa > 1:566604/1‑90 (MQ=255) ttcttcACCGGCAT‑GGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTTGTGCGGGAGGGGaaaac > 1:694319/1‑88 (MQ=255) gCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCAAGCGTGACGGGAAAACGCCtttttt > 1:182116/1‑87 (MQ=255) ttattTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGGCGGGAAAACGCATTTGCTAAAAAAGGa > 1:208808/1‑90 (MQ=255) ttattTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGGGACGGGGAACCGCATTTTCTAAAAATGGa > 1:294833/1‑90 (MQ=255) cGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGGGGCGGGAAACCGCATTTTCTTAAAAAGGAAAAAGa > 1:636387/1‑90 (MQ=255) tttGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGACGGTAAACCGCATTTGCTAAAAATGGAAAACGACAGCACGctgctg < 2:791974/90‑1 (MQ=255) aCGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGACGGTAAACCGCATTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCaa < 1:511330/90‑1 (MQ=255) aCGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGACGGTAAACCGCATTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCaa < 2:450964/90‑1 (MQ=255) cAGCAAACTGTTCAGTGGCGGTCATGCGTGACGGGAAACCGCATTTGCTAAAAAAGGAAAAAAAAAGCACGCTGCTGCCCTCAATGCttt > 1:781053/1‑90 (MQ=255) cTGTTCAGTGGCGGTCATGCGTGGCGGGAAAACGCATTTTTTAAAAAAAGAAAAAAAAAGCACGCCGCTGGCTTCAATGCTTTTTGCGcc > 2:629434/1‑90 (MQ=255) tGTTCAGTGGCGGTCATGCGTGACGGTAAACCGCATTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCa < 1:488779/90‑1 (MQ=255) gTTCAGTGGCGGTCATGCGTGGCGGGAAACCGCATTTTCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTTCGCGccca > 1:104293/1‑88 (MQ=255) ggTCATGCGTGGCGGGAAAACCCCTTTTCTAAAAAAGGAAAACGACAGCACGCCGCTGCCCTCCATGATTTTGGCGCCCACGCGCGaaag > 2:768076/1‑88 (MQ=255) gACGGTAAACCGCATTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCGaa < 1:777208/90‑1 (MQ=255) | CTTCTTCACCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGACGGTAAACCGCATTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCGAA > NZ_CP009273/2141128‑2141298 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |