| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,012,922 | 0 | T | G | 52.2% | 22.9 / 33.1 | 23 | S135A (TCG→GCG) | ssnA | putative aminohydrolase SsnA |
| Reads supporting (aligned to +/- strand): ref base T (0/11); new base G (12/0); total (12/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.40e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.59e-05 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACAGCGGACTGATTTGTTCCCTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCGCTCTCCACATTGCGCGA‑CG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAACTACTGACCGTAACAAC > NZ_CP009273/3012833‑3013002 | aCAGCGGACTGATTTGTTCCCTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGt < 1:646280/90‑1 (MQ=255) aGCGGACTGATTTGTTCCCTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCg < 2:264892/90‑1 (MQ=255) gCGGACTGATTTGTTCCCTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGGCgg > 1:588250/1‑89 (MQ=255) ggACTGATTTGTTCCCTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGt < 1:534309/85‑1 (MQ=255) ggACTGATTTGTTCCCTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGGGGctc > 1:483134/1‑90 (MQ=255) tAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCGCTCTCCACATTGCGCGA‑CG‑CATTTTTaaa < 1:216095/90‑1 (MQ=255) aCATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCGCTCTCCACATTGCGCGA‑CG‑CATTTTTAAAAGTTGGCCTgcgc < 1:497667/90‑1 (MQ=255) aCATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCGCTCTCCACATTGCGCGA‑CG‑CATTTTTAAAAGTTGGCCTgcgc < 1:460054/90‑1 (MQ=255) tCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGGCGCTCTCCCCATTGCGCGG‑CG‑CATTTTTTAAAATTGGGCTGcgcgcg > 1:224445/1‑90 (MQ=255) ggTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGGGGCCCTCCCCCCTTCGCGG‑CG‑CCTTTTTTTAAAATGGGCTGcgcgcggt > 2:242006/1‑88 (MQ=255) ggTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGGCGCTCTCCCCATTTTGCGACCC‑CTTTTTTAAAAATTGG‑CTGcgggcggg > 1:613487/1‑88 (MQ=255) gTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGGGGCCCTTCCCATTTCGCGG‑CG‑CATTTTTTAAAATTGGCCTGcgcgcgggg > 2:497667/1‑87 (MQ=255) tATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGGCGCTCTCCCCCATTCGCGA‑CGCCCTTTTTAAAAATTGGCC‑GCGCGCGGTGAc > 1:82475/1‑90 (MQ=255) aTCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCGCTCTCCACATTGCGCGA‑CG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGAcc < 2:700885/90‑1 (MQ=255) aTCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCGCTCTCCACATTGCGCGA‑CG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGAcc < 1:600541/90‑1 (MQ=255) tCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCGCTCTCCACATTGCGCGA‑CG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCt < 2:82475/90‑1 (MQ=255) tCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCGCTCTCCACATTGCGCGA‑CG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCt < 1:795983/90‑1 (MQ=255) aTCACCATGCCTCTCCGGCGTATATCGGCGGGGCGGGCTCCCCCTTTCGCCG‑CGCCTTTTTTTAAAATTGGCC‑GGGCGGGGTGACCCGtt > 2:598096/1‑90 (MQ=255) aTCACCATGCCTCTCCGGCGTATATCGGCGGGGCGGCCTCCCCCTTTCGCGG‑CGCCTTTTTTTAAAATTGG‑CTGCGCGCGGGGGCCTGtt > 2:148338/1‑90 (MQ=255) ccATGCCTCTCCGGCGTATATCGGCGGGTCGCTCTCCACATTGCGCGA‑CG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGa < 2:592498/90‑1 (MQ=255) ctcCGGCGTATATCGGCGGGGCGCTCTCCCCATTGCGCGA‑CG‑CCTTTTTTAAAATTGGCCCGCGCGCGGTGACCCGTTTTTTAACTACTg > 1:52988/1‑90 (MQ=255) ccGGCGTATATCGGCGGGGCGCTCTCCCCCTTTCGCGG‑CGCCTTTTTTTAAATTTGGCC‑GCGCGCGGGGGCCCGTTTTTTAAATACTTAc > 2:195196/1‑90 (MQ=255) ccGGCGTATATCGGCGGGGCGCCCTCCCCCATTCGCGG‑CG‑CCTTTTTTTAAAATTGCCCGCGCGCGGGGGCCCGTTTTTTAAATAATGAc > 2:178066/1‑90 (MQ=255) atCGGCGGGTCGCTCTCCACATTGCGCGA‑CG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAACTACTGACCGTaacaac < 1:148338/90‑1 (MQ=255) atCGGCGGGTCGCTCTCCACATTGCGCGA‑CG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAACTACTGACCGTaacaac < 1:598096/90‑1 (MQ=255) | ACAGCGGACTGATTTGTTCCCTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCGCTCTCCACATTGCGCGA‑CG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAACTACTGACCGTAACAAC > NZ_CP009273/3012833‑3013002 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |