| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,029,116 | 0 | A | C | 61.5% | 3.3 / 11.1 | 13 | G175G (GGT→GGG) | prfB | peptide chain release factor 2 |
| Reads supporting (aligned to +/- strand): ref base A (5/0); new base C (0/8); total (5/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.77e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.13e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGAT > NZ_CP009273/3029029‑3029186 | tttACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATAcc > 2:596280/1‑90 (MQ=255) aGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCa > 2:635607/1‑90 (MQ=255) gAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCg > 1:133901/1‑90 (MQ=255) gCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGActc > 2:27708/1‑90 (MQ=255) gTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGAtt > 2:452374/1‑90 (MQ=255) aaGCGTAACCGCGGGAGTTTTTGATCGCCAGGATTTTAATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCAc < 1:312133/90‑1 (MQ=255) ccGGAATTTTTGACCGAAACGAATTAAAACCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGcc < 2:493600/90‑1 (MQ=255) cGGAGTTTTTGATCGTCAGGTATTTAATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGccc < 1:279416/90‑1 (MQ=255) cGGAGTTTTTGATCGTCACGTTTTTAATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGccc < 1:19974/90‑1 (MQ=255) attttttGATCGTCCCGTATTTAACCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAgc < 2:428306/87‑1 (MQ=255) tttttGTCCGCCAGGGTTTAAATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAgcgc < 1:17338/89‑1 (MQ=255) tttttGATCGTCAGGTATTAAATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAgcgc < 1:132773/89‑1 (MQ=255) tGATCGTCACGTTTTTAATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAACCCACGCGATTCTGCCCAGCGCAGat < 1:158471/90‑1 (MQ=255) | TTTACGCACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTTAATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGAT > NZ_CP009273/3029029‑3029186 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |