| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,401,190 | 0 | C | G | 55.0% | 10.2 / 23.7 | 20 | G75G (GGC→GGG) | panF | sodium/pantothenate symporter |
| Reads supporting (aligned to +/- strand): ref base C (3/6); new base G (11/0); total (14/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.17e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.51e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATTGTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCG > NZ_CP009273/3401101‑3401276 | aTTGTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACgggctgggc < 2:712568/90‑1 (MQ=255)aTTGTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACgggctgggc < 1:17895/90‑1 (MQ=255)aTTGTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACgggctgggc < 1:667566/90‑1 (MQ=255) ccGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGGGCCGGTTTTAAAAAAGGGGTGGGGGGGGGATTTTTGGCGATGATTc > 1:93398/1‑90 (MQ=255) gTGCCAGTTCGTTTATCGGCGGGGCCGGAGCGGATTATAAAAAAGGGCTGGGGGGGGGATTTTTGGCGGGGATTCGGCTTACTGCCGTCt > 2:430096/1‑90 (MQ=255) gTGCCAGTTCGTTTATCGGCGGGCCCGGGGCTGCTTATAAATAAGGGGTGGGGTGGGGGTTGCTGGCGATGATTCAGCTTCCCGCAGGCt > 1:447895/1‑90 (MQ=255) gTGCCAGTTCGTTTATCGGCGGGCCCGGAGGTGCTTTTAAATACGGGCTGGGCTGGGGATTTCTGGCGATGGTTCAGCTTCCTGCAGTCt > 1:437167/1‑90 (MQ=255) gTGCCAGTTCGTTTATCGGCGGGCCCGGAGCTGCTTATAAATAAGGGCTGGGGTGGGGATTGCTGGGGGTGGTTTAGCTTCCTGCAGTCt > 2:75946/1‑90 (MQ=255) gTGCCAGTTCGTTTATCGGCGGGCCAGGGGCTGCTTTTAAAAAAGGGCTGGGGTGGGGATTGCTGGGGGTGATTCAGCTTCCTGCAGTCt > 1:736039/1‑90 (MQ=255) gTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCt < 1:66240/90‑1 (MQ=255) gTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGGATTGCTGGGGATGATTCAGCTTCCTGCAGTCt > 1:326759/1‑90 (MQ=255) gCCAGTTCGTTTATCGGCGGGCCCGGGGGCGCTTTTAAAAAAGGGGCGGGGTGGGGGTTGCTGGGGGTGATTTAGCCTCCTGCCGGCTgg > 2:267455/1‑90 (MQ=255) gCCAGTTCGTTTATCGGCGGGCCAGGGGGTGCTTTTAAATAAGGGCTGGGGTGGGGATTTCTGGGGGTGGTTTAGCTTTCTTCCGTCTgg > 2:208029/1‑90 (MQ=255) cAGTTCGTTTATCGGCGGGCCAGGGGCTGCTTTTTAAAAAGGGGTGGGGTGGGGGTTTGTGGGGGTGGTTTTGCTTTCTGCCGTCTgggt > 2:463097/1‑88 (MQ=255) ttCGTTTATCGGCGGGCCCGGGGGCGCTTATAAATAAAGGCTGGGGTGGGGATTGCTGGGGCGGATTTAGCTTTCTGCGGTTTGGGtttt > 1:482794/1‑89 (MQ=255) tCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCa < 2:482794/90‑1 (MQ=255) cGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCAc < 2:736039/90‑1 (MQ=255) gTTTATCGGCGGGCCCGGGGGTGCTTTTAAAAAAGGGGTGGGGTGGGGGTTGCTGGGGGGGGTTTCGCTTTCTTCCGGCTGGCTTTTACt > 2:310688/1‑90 (MQ=255) gTTTATCGGCGGGCCCGGGGCTGCTTATAAATAAGGGGTGGGGTGGGGGTTTCTGGCGGTGGTTTAACTTCCTGCAGTCTGGGTTTTACt > 2:151584/1‑90 (MQ=255) tttATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTc < 2:437167/90‑1 (MQ=255) tATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCgg < 1:541386/90‑1 (MQ=255) ctgctTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGt > 1:226729/1‑90 (MQ=255) ttATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGc < 1:121657/90‑1 (MQ=255) gggctgggctGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTgcg > 1:221891/1‑90 (MQ=255) ggctgggctgGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATtct > 1:288723/1‑64 (MQ=255) ggctgggctgGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATtct < 2:288723/64‑1 (MQ=255) ggctgggctgGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTgcgc < 2:585090/90‑1 (MQ=255) ggctgggctgGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTgcgc < 2:226729/90‑1 (MQ=255) ggctgggctgGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTgcgc > 2:139765/1‑90 (MQ=255) ggctgggctgGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTgcgc < 1:75946/90‑1 (MQ=255) ggctgggctgGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTgcgc > 1:585090/1‑90 (MQ=255) tgggctgggTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGcgcc < 2:233750/90‑1 (MQ=255) gggctgggTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGcgccg > 1:104939/1‑90 (MQ=255) | ATTGTGCTGGCGATGACGCTCACCGCGACCTATATCAGTGCCAGTTCGTTTATCGGCGGGCCAGGAGCTGCTTATAAATACGGGCTGGGCTGGGTATTGCTGGCGATGATTCAGCTTCCTGCAGTCTGGCTTTCACTCGGTATTCTCGGCAAGAAGTTTGCGATTCTTGCGCGCCG > NZ_CP009273/3401101‑3401276 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |