| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,726,640 | 0 | T | A | 52.4% | 9.2 / 28.0 | 22 | N360K (AAT→AAA) | xylG | D‑xylose ABC transporter ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (1/9); new base A (11/0); total (12/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.40e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.06e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTC > NZ_CP009273/3726557‑3726716 | cAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTAcc < 2:538714/90‑1 (MQ=255) gCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCa < 1:281200/90‑1 (MQ=255) cccAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCAc < 1:363250/90‑1 (MQ=255) ttGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAAt > 1:289914/1‑90 (MQ=255) ttGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAAAATTACCCCCGCCGCACTCAATAAAt > 1:616950/1‑90 (MQ=255) ttGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGGAAAAAAATTACCCCCGCCGCACTCAATaaaa > 1:723133/1‑89 (MQ=255) ttGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGGAAAAAAATTACCCCCCCCGCACCCAATaaaa > 1:37827/1‑89 (MQ=255) tGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGGAAAAAAATTAACCTCGCCCCCCCCaaaaaaat > 1:752316/1‑88 (MQ=255) tGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGGTGGGGAAAAAAATAACCCCGCCCCCCCCAAAAAAtt > 2:596382/1‑90 (MQ=255) ggTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTTGTAAAAAAATTACCCCCCCCCCCCCCAATAAAATTACCgg > 1:538683/1‑90 (MQ=255) gTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGt < 2:380906/90‑1 (MQ=255) ccccGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGGAAAAAAATTACCCCCCCCCCCCTCCAAAAAATTTCCCGTgg > 1:385334/1‑90 (MQ=255) gcgACGGCATCGTTCCGGTAATGGCGGTTGGGAAAAAAAATAACCCCCCCCCCCCCAAAAAAATTACCCGGGGGATTAACCAGCTtgatg > 2:210309/1‑90 (MQ=255) gACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAACATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGAc < 2:538683/90‑1 (MQ=255) cGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGAcgc < 2:200323/90‑1 (MQ=255) tCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCag < 1:225610/90‑1 (MQ=255) ccGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCaa < 2:385926/90‑1 (MQ=255) cGGTAATGGCGGTTGGGAAAAAAATTACCCCCCCCCCCCTCAATAAATTTACCGGGGGGATTAGCCAGCTTGATGACGCGGCCGAGCaaa > 2:295464/1‑90 (MQ=255) ggTAATGGCGGTTGGGAAAAAAATTACCCCCGCCCCCCTCCATAAATTTTCCGGGGGGATTAGCCAGCTTGTTGACGCGGCAGAGCaaaa > 2:175980/1‑90 (MQ=255) tGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTa < 2:176495/90‑1 (MQ=255) tGGCGGTTGGTAAAAAAATTACCCCCCCCCCCCTCAATAAAATTACCGGGGGGATTAACCAGCTTGATGACGCGGCAGAGGaaaaaaata > 2:266339/1‑86 (MQ=255) cGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTc < 1:266339/90‑1 (MQ=255) | CAAGCCATCGCCCAGGGGATTGCGATGGTCCCCGAAGACAGAAAGCGCGACGGCATCGTTCCGGTAATGGCGGTTGGTAAAAATATTACCCTCGCCGCACTCAATAAATTTACCGGTGGCATTAGCCAGCTTGATGACGCGGCAGAGCAAAAATGTATTC > NZ_CP009273/3726557‑3726716 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |