Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,321,6170CT54.5% 4.9 / 11.1 11I433I (ATC→ATT) proPglycine betaine/L‑proline transporter ProP
Reads supporting (aligned to +/- strand):  ref base C (0/5);  new base T (5/1);  total (5/6)
Fisher's exact test for biased strand distribution p-value = 1.52e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

ACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGTTGGTTTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCCGTT‑GAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAA  >  NZ_CP009273/4321531‑4321692
                                                                                      |                                                                            
aCGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGTTGGTTTAATCAcc                                                                           <  1:397352/90‑1 (MQ=255)
     ggcggcCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGGGGGTGGGTTTATTACCcgcgg                                                                      >  1:459946/1‑89 (MQ=255)
                   tCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGTTGGTTTAATCACCGGCGTAACCATGAAAgaga                                                        <  2:246142/90‑1 (MQ=255)
                                       atgatgCCTGCCTATTACCTGATGGTAGTGGCGGTGGTTGGTTTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCCGTT‑Gaaa                                   <  1:458580/90‑1 (MQ=255)
                                          atgCCTGCCTATTACCTGATGGTAGTGGCGGGGGGTGGGTTTATTACCCGCGGAACCCTGAAAAAGAAGGGAAAACGTCCGGT‑GGAAggg                                >  1:620908/1‑89 (MQ=255)
                                             caggcctATTACCTGATGGTAGTGGCGGTGGTTGGTTTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCCGTT‑GAAAGGTGCg                             <  1:784409/87‑1 (MQ=255)
                                              ctgcctATTACCTGATGGTAGTGGCGGGGGGTGGTTTAATTACCCGCGGAAACATGAAAAAAAAGGGAAAACGTCCCTTGGAAAgggggg                             >  1:449868/1‑86 (MQ=255)
                                                         cTGATGGTAGTGGCGCTGGTTGGTTTAATTACCGGCCTAACCCTGAAAAAGACGCCCAATCGTCCGTT‑GAAAGGTGCCAAACCGgcggcg                 <  2:438195/90‑1 (MQ=255)
                                                          tGATGGTAGTGGCGGGGGGTGGGTTTATTAACCGCGGGACCCTGAAAAAGAAGGGAAAAAGTTCGTT‑GTAAGGGGGGGCACccgcggggg                >  2:784409/1‑87 (MQ=255)
                                                           gATGGTAGTGGCGGGGGGTGGGTTTATTACCCGCGGAAACATGAAAAAGAAGGGAAAACGGCCGTT‑GGAAAGGGGGGCACccgcgggggc               >  2:175954/1‑86 (MQ=255)
                                                                        gtggtTGGTTTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCCGTT‑GAAAGGTGCGACACCGGCGGCGTCAGATATACAGGaa  <  2:301833/90‑1 (MQ=255)
                                                                                      |                                                                            
ACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGTTGGTTTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCCGTT‑GAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAA  >  NZ_CP009273/4321531‑4321692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑