| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 108,785 | 0 | A | C | 57.1% | 14.5 / 20.8 | 21 | V101G (GTA→GGA) | zapD | cell division protein ZapD |
| Reads supporting (aligned to +/- strand): ref base A (7/2); new base C (0/12); total (7/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.10e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.98e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCACGCCAATCCAGGTCTG > NZ_CP009273/108706‑108864 | gATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAAAAATTGCCCGATACGCGGCGCGAAAATTAATACGCCCCccgc < 2:176658/90‑1 (MQ=255) gCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCcgccgc > 1:579648/1‑90 (MQ=255) gTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTa > 1:448475/1‑90 (MQ=255) tGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAActgc > 2:605591/1‑90 (MQ=255) agagCAATCAAACGATCTCCCCGCAAAAATTCCCCGATCCGCGGCGCGAAAATTAATCCCCCCCCCGCCGCTTTTAACTGCTGAATTAAt < 2:649213/90‑1 (MQ=255) aaTAAAACAATCTCCCCGAAAAAATGCCCCGATACGCGGCGCGAAAATAAATCCCCCCCCCGCCGCTTTTAACTGCTGAATTAATGCTTc < 2:96565/90‑1 (MQ=255) aaCGATCTTCACGCAGAAATTGCCCGATACGCGGCGGGGAAATTAATCCCCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATAc < 1:409525/90‑1 (MQ=255) aaCGATCTTCACGCAAAAATTGCCCGATACGCGGCGCGGAAATTAATCCGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATAc < 1:7416/90‑1 (MQ=255) aaCGATCTCCCCGCAGAAATTCCCCGATCCGCGGCGGGAAAATTAATCCCCCCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATAc < 2:448475/90‑1 (MQ=255) aaCGATCTCCACGCAAAATTTGCCCGATCCGCGGCGGGGAAATTAATCCGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATAc < 2:176097/90‑1 (MQ=255) tCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTgg > 2:608713/1‑90 (MQ=255) aGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACg > 2:160425/1‑90 (MQ=255) aaaaTTGCCCGATACGCGGCGCGAAAATTAATCCCCCCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGc < 1:706072/89‑1 (MQ=255) gCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCa > 2:425416/1‑90 (MQ=255) cgaaTACGCGGCGCGAAAATTAATCCCCCCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCACg < 1:776649/87‑1 (MQ=255) cGATCCGCGGCGGGAAAATAAACCCCCCCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCACGc < 2:35093/90‑1 (MQ=255) gcggcgggaAAATAAATCCCCCCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCACGCCAATcc < 2:447871/90‑1 (MQ=255) gcggcgcgaAAATTAATCCCCCCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCACGCCAATcc < 1:605591/90‑1 (MQ=255) gcggAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCACGCCAATCCAGGTc > 1:412893/1‑90 (MQ=255) ggaaaaTTAATACCCCCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCACGCCAATCCAGGTCt < 1:741983/87‑1 (MQ=255) gaaaaTTAATCCGCCCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCACGCCAATCCAGGTCTg < 1:299162/88‑1 (MQ=255) | GATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCACGCCAATCCAGGTCTG > NZ_CP009273/108706‑108864 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |