Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273536,0140TG58.3% 3.9 / 10.6 12intergenic (+50/‑7)allB/ybbYallantoinase AllB/uracil/xanthine transporter
Reads supporting (aligned to +/- strand):  ref base T (1/4);  new base G (7/0);  total (8/4)
Fisher's exact test for biased strand distribution p-value = 1.01e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTGT  >  NZ_CP009273/535942‑536100
                                                                        |                                                                                      
ttATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAAttt                                                                       <  1:189771/90‑1 (MQ=255)
             tCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCCGTTAAGGGGGGTTTATTTTCCATTTTTCAGGCAGCcgc                                                          >  1:197731/1‑90 (MQ=255)
                    tAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGcc                                                   <  1:250733/90‑1 (MQ=255)
                     aaTCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCCTTTTCCGGGTAAGGGGGGGTTTTGTTCAATTTTTCCGGCAGCCGCGGAAGCCt                                                  >  2:250733/1‑90 (MQ=255)
                          ggCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGGTTAGGGGGGTTTTTGTTTAATTTTTCAGTCAGCCGCGAAAGCCTGtttt                                             >  2:248771/1‑88 (MQ=255)
                               cTGCAATGCCCGTCCTTGCGGCGGGGATTCTCCGGGTAAGGGGGGTTTTTTTTTTATTTTTTAGTGAGCCCCGGAAACCTGTTTTCAggg                                        >  1:356255/1‑89 (MQ=255)
                               cTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCCGTTTAGGGGGGTTTTTGTTTAATTTTTTAGTCAGCCGCGGAAACCCGTTTTTAggg                                        >  1:593217/1‑89 (MQ=255)
                                      gCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGt                                 <  1:297975/90‑1 (MQ=255)
                                         cGTCCTTGCGGCGGGCATTATTCGGTTAAGGGGGGTTTTTGTTTAATTTTTCAGTCGGCCGGGGAAGCCTGTTTTCCGGGTTTTTGTGGt                              >  1:565428/1‑90 (MQ=255)
                                                gcggcgGGCATTCTCCGGTTAAGGGGGGTTTATGTTTAATTTTTCAGGCAGCCGCGAAAGCCTGTTATCAGGATTTTAGTGGGTtttttt                       >  2:297975/1‑90 (MQ=255)
                                                               cGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAacac        <  1:88114/90‑1 (MQ=255)
                                                                     aGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTtgt  >  2:239426/1‑90 (MQ=255)
                                                                        |                                                                                      
TTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTGT  >  NZ_CP009273/535942‑536100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑