| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 700,041 | 0 | T | G | 55.6% | 14.7 / 19.4 | 19 | G214G (GGT→GGG) | nagE | PTS N‑acetyl glucosamine transporter subunit IIABC |
| Reads supporting (aligned to +/- strand): ref base T (1/7); new base G (10/0); total (12/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.51e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.11e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATCCCAACCGGTCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGG‑TTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCACCGCGGGGATGTTCATGTCCGGCTTCTTCCCGATCATGATGTT > NZ_CP009273/699952‑700115 | aTCCCAACCGGTCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGG‑TTTTCCACGGt < 2:460439/90‑1 (MQ=255) aCCGGTCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGGACGG‑GTTTTCCCGGGGACAtt > 1:308988/1‑90 (MQ=255) ggTCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGGACGG‑TTTTTCACGGTGGCATTAAc > 1:6691/1‑90 (MQ=255) gTCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGG‑TTTTCCACGGTGACATTAAcc < 1:261542/90‑1 (MQ=255) cATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGG‑TTTTCCACGGTGACATTAACCGCTTc < 1:406744/90‑1 (MQ=255) cTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGCACGG‑GTTTCCCCGGGGACATTAACCGCTTCTATTCCggg > 1:414973/1‑89 (MQ=38) acacCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGGCTTTTCAACGG‑GGGATTAAACGGCTCTATGCCGGTGGCg > 1:251440/1‑90 (MQ=255) acCATCGCCTGTTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGG‑TTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGc < 2:152217/90‑1 (MQ=255) acCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGGACGG‑GTTTTCCCGGGGACATTAACCGCCTCTATGCCGGGggcggc > 1:106170/1‑90 (MQ=255) gCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGG‑GTTTTCACGGGGGGATTAAACGCTTCTATTCCCGTGACGGCGCcccg > 1:369537/1‑90 (MQ=255) gCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTAAGG‑GTTTCCACGGGGGCCTTAACCGCTTCTATACCGGAGGCGGCACcgcg > 2:372618/1‑90 (MQ=25) gTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGG‑TTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCACCGCGGGGAt < 1:150917/90‑1 (MQ=255) gTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGG‑GTTTCCCCGGGGACAATAAACGCCTCTATTCCCGTGGCGGCACCCCcggggg > 1:552837/1‑88 (MQ=255) aGATTGGTGAATTCACCAACGCGGCGGGTACGG‑GTTTTCCCGGGGACATTAACCGCTTCTATGCCGGGGGCGGCACCGCGGGGGTggtta > 1:69543/1‑86 (MQ=255) ggTGAATTCACCAACGCGGCGGGGACGG‑GTTTTCACGGGGGCATTAACCGCTTCTATTCCGGGGACGGCACCGCGGGGGTGGTTATTTcc > 2:63460/1‑90 (MQ=255) ggTGAATTCACCAACGCGGCGGGGACGG‑ATTTCCACAAGGACAGTAAACACCTCCATTCCCCTGGCGGCACCCCGGGGGAGTTCCTGttc > 1:490191/1‑88 (MQ=255) cAACGCGGCGGGTACGG‑TTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCACCGCGGGGATGTTCATGTCCGGCTTCTTccc < 1:417264/90‑1 (MQ=255) gcggGTACGG‑TTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCACCGCGGGGATGTTCATGTCCGGCTTCTTCCCGATCatg < 1:63460/90‑1 (MQ=255) ggaCGG‑GTTTCCACGGGGACATTAACCGCTTCTATGCCGGGGACGGCCCCGCGGGGGGGGTCATGTCCGGCTTCTTCCCGATCATGgtgt > 1:74866/3‑90 (MQ=255) tACGG‑TTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCACCGCGGGGATGTTCATGTCCGGCTTCTTCCCGATCATGATGtt < 2:308988/90‑1 (MQ=255) | ATCCCAACCGGTCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGG‑TTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCACCGCGGGGATGTTCATGTCCGGCTTCTTCCCGATCATGATGTT > NZ_CP009273/699952‑700115 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |