Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,002,5710AG57.1% 9.2 / 15.0 14I272V (ATC→GTC) yedEselenium metabolism membrane protein YedE/FdhT
Reads supporting (aligned to +/- strand):  ref base A (1/5);  new base G (8/0);  total (9/5)
Fisher's exact test for biased strand distribution p-value = 3.00e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GTTCCGCGATATGTGGATCACCGGACGTACCCATATGGCGAAAGCAATCATTATCGGTATGGCGGTGAGTGCCATCGGGATCTTCAGTTACGTACAGTTAGGCGTTGAACCCAAAATCATGTGGGCGGGACCAAACGCGGTAATT  >  NZ_CP009273/2002492‑2002636
                                                                               |                                                                 
gTTCCGCGATATGTGGATCACCGGACGTACCCATATGGCGAAAGCAATCATTATCGGTATGGCGGGGGGGGCCATCGGGGTCTTCAGTTa                                                         >  2:22961/1‑90 (MQ=255)
   ccGCGATATGTGGATCACCGGACGTACCCATATGGCGAAAGCAATCATTATCGGTATGGCGGTGAGGGCCCTCGGGGTCTTTAGTTACGt                                                      >  2:24824/1‑90 (MQ=255)
                  cACCGGACGTACCCATATGGCGAAAGCAATCATTATCGGTATGGCGGTGAGTGCCATCGGGGTCTTCAGTTACGTACAGTTAGGCGTTGa                                       >  2:426532/1‑90 (MQ=255)
                     cGGACGTACCCATATGGCGAAAGCAATCATTATCGGTATGGCGGTGAGGGCCATCGGGGTCTTCAGGTACGTAAAGTTAGGGGTTGaaac                                    >  2:34697/1‑88 (MQ=255)
                                    ggCGAAAGCAATCATTATCGGTATGGCGGTGAGTGCCATCGGGATCTTCAGTTACGTACAGTTAGGCGTTGAACCCAAAATCATGTGGGc                     <  1:24824/90‑1 (MQ=255)
                                     gCGAAAGCAATCATTATCGGTATGGCGGTGGGGGGCACCGGGGTCTTCAGTTACGTAAAGTTAGGGGTTGAACCCAAAAACATGgggggg                    >  1:405976/1‑88 (MQ=255)
                                     gCGAAAGCAATCATTATCGGTATGGCGGTGAGGGCCATCGGGGTCTTCAGTTACGTACAGTTAGGCGTTTAACCCAAAAACATGTggggg                    >  2:80876/1‑88 (MQ=255)
                                     gCGAAAGCAATCATTATCGGTATGGCGGGGGGGGCCCTCCGGGTCTTTCATTACGTACAGTTAGGGGGTGAACCCAAAATCATGTggggg                    >  1:277927/1‑88 (MQ=255)
                                     gCGAAAGCAATCATTATCGGTATGGCGGGGAGGGCCATCGGGGTCTTCAGTTACGTACAGTTAGGCGTTGAAACCAAAATCATGTGGGCg                    >  2:161550/1‑90 (MQ=255)
                                            cAATCATTATCGGTATGGCGGTGAGTGCCATCGGGATCTTCAGTTACGTACAGTTAGGCGTTGAACCCAAAATCATGTGGGCGGGACCaa             <  2:173106/90‑1 (MQ=255)
                                                       ggTATGGCGGTGAGTGCCATCGGGATCTTCAGTTACGTACAGTTAGGCGTTGAACCCAAAATCATGTGGGCGGGACCAAACGCGGTAAtt  <  1:114136/90‑1 (MQ=255)
                                                       ggTATGGCGGTGAGTGCCATCGGGATCTTCAGTTACGTACAGTTAGGCGTTGAACCCAAAATCATGTGGGCGGGACCAAACGCGGTAAtt  <  1:259726/90‑1 (MQ=255)
                                                                    gTGCCATCGGGATCTTCAGTTACGTACAGTTAGGCGTTGAACCCAAAATCATGTGGGCGGGACCAAAcgc         >  1:247438/1‑70 (MQ=255)
                                                                    gTGCCATCGGGATCTTCAGTTACGTACAGTTAGGCGTTGAACCCAAAATCATGTGGGCGGGACCAAAcgc         <  2:247438/70‑1 (MQ=255)
                                                                               |                                                                 
GTTCCGCGATATGTGGATCACCGGACGTACCCATATGGCGAAAGCAATCATTATCGGTATGGCGGTGAGTGCCATCGGGATCTTCAGTTACGTACAGTTAGGCGTTGAACCCAAAATCATGTGGGCGGGACCAAACGCGGTAATT  >  NZ_CP009273/2002492‑2002636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑