| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,794,508 | 0 | T | G | 53.3% | 9.8 / 15.9 | 15 | V62G (GTA→GGA) | nrdI | class Ib ribonucleoside‑diphosphate reductase assembly flavoprotein NrdI |
| Reads supporting (aligned to +/- strand): ref base T (1/6); new base G (8/0); total (9/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.40e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.72e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTCGCGGCGTTATTGCTTCTGGTAATCGCAACTTTGGTGAGG > NZ_CP009273/2794423‑2794588 | gAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGGACGGGTGGGGCGGGGCCCCGACAGGGAAtt > 1:456907/1‑90 (MQ=255) ggATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGGACGGCTGGGGCGGGGCCACGACAGGGAATTCGCt > 2:155907/1‑90 (MQ=255) aGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGc < 2:319630/90‑1 (MQ=255) ccTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGGGCGGGGCCCCCGCAGGGAATTCGCTTTTTTAACGGCGGGcac > 1:168242/1‑90 (MQ=255) cTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGGGCCACGACAGGGAATTCGCTTTTTAAACGACGAGCACAACCGGGCg > 2:255567/1‑90 (MQ=255) tGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGGGCGGGGCCCCGGCAGGGAAATTGCGTTTTTAAAGACGGGCACCACCCGGGGt > 1:616130/1‑90 (MQ=255) gCCCTCTTACGGCGGCGGCGGTACGGCTGGCTCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTcg > 2:249996/1‑90 (MQ=255) gCCCTCTTACGGCGGCGGCGGGACGGCTGGGGGGGGGCCCCGACAGGGAAATCGCTTTTTTAAAGACGAGCACAACCGGGGGTTGCTTcg > 1:206767/1‑90 (MQ=255) cccTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTcgc < 1:148352/90‑1 (MQ=255) cccTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTcgc < 1:155907/90‑1 (MQ=255) cccctTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTcgcg < 2:450742/87‑1 (MQ=255) ggcggcggTACGGCTGGCGCGGGGCCACGACAGGGAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTCGCGGCGTTTTTGCt > 2:51355/1‑90 (MQ=255) ggcggcggTACGGCTGGCGCGGGGCCACGACAGGGAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTCGCGGCGTTATTTCt > 2:375946/1‑90 (MQ=255) gTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTCGCGGCGTTATTGCTTCTGGTa < 2:542375/90‑1 (MQ=255) cACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTCGCGGCGTTATTGCTTCTGGTAATCGCAACTTTGGTGAgg < 2:133642/90‑1 (MQ=255) | GAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTCGCGGCGTTATTGCTTCTGGTAATCGCAACTTTGGTGAGG > NZ_CP009273/2794423‑2794588 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |