| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,775,223 | 0 | T | G | 57.1% | 4.9 / 11.1 | 15 | T234P (ACC→CCC) | cysE | serine O‑acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (2/4); new base G (8/0); total (11/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.50e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.75e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTTGAAATGCTGGTCCATATCCATTGATGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGTGGTATGCGGCGGCACCGGTTGCAGCACCACGGAACCTGCGCCAATCTTCGCGCCGCGCCCAACTTCAATAT > NZ_CP009273/3775140‑3775291 | gTTGAAATGCTGGTCCATATCCATTGATGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGTGGGAttggg > 2:505005/1‑86 (MQ=255)gTTGAAATGCTGGTCCATATCCATTGATGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGTGGGATgcgg > 2:517044/1‑90 (MQ=255)gTTGAAATGCTGGTCCATATCCATTGATGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGGGGTATgcgg > 2:166663/1‑90 (MQ=255)gTTGAAATGCTGGTCCATATCCATTGATGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGGGGGATgtgg > 1:98902/1‑90 (MQ=255) gAAATGCTGGTCCATATCCATTGATGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGTGGTATGcggcgg < 2:65600/90‑1 (MQ=255) tGGTCCATATCCATTGATGGCTTATCGCTGTCTGGTTTTCCGACAATACGAGCCGGAACGCCAGCGGCGGTGGGATGGGGGGGCGCCGGt > 1:161164/1‑90 (MQ=255) tatCCATTGATGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGTGGTATGCGGCGGCACCGGTTgcagca < 1:297721/90‑1 (MQ=255) tatCCATTGATGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGTGGTATGCGGCGGCACCGGTTgcagca < 1:397422/90‑1 (MQ=255) cATTGATGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGTGGGATGCGGCGGCACCGGGTGCAGcaccac > 2:323440/1‑90 (MQ=255) cATTGATGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGGGGGGTGGGGCGGCACCCGGTGCCGcacccc > 1:426369/1‑88 (MQ=255) aTTGATGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGTGGTATGCGGGGGGACCAGTTGCCGCACCACg > 1:475540/1‑90 (MQ=255) tGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGGGGGATTCGGCGGCACCCGTTGCAGCCCCACGGGAcc > 2:84610/1‑90 (MQ=255) gCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGTGGCATGGGGGGGGCCCGGGTGCAGCACCCCGGAACCtt > 2:231569/1‑89 (MQ=255) tGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGTGGGATGCGGCGGCACCCGTTGGAGCACCCCGGGACCTGCGCCCATCt > 2:280744/1‑90 (MQ=255) ccGGAACGCCAGCGGCGGTGGTATGCGGCGGCACCGGTTGCAGCACCACGGAACCTGCGCCAATCTTCGCGCCGCGCCCAACTTCAatat < 2:74475/90‑1 (MQ=255) | GTTGAAATGCTGGTCCATATCCATTGATGGCTTATCGCTGTCTGGTTTACCGACAATACGAGCCGGAACGCCAGCGGCGGTGGTATGCGGCGGCACCGGTTGCAGCACCACGGAACCTGCGCCAATCTTCGCGCCGCGCCCAACTTCAATAT > NZ_CP009273/3775140‑3775291 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |