| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,142,237 | 0 | T | G | 57.9% | 6.4 / 15.0 | 19 | T264P (ACC→CCC) | ppc | phosphoenolpyruvate carboxylase |
| Reads supporting (aligned to +/- strand): ref base T (4/4); new base G (11/0); total (15/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.81e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.12e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTTCAGAAACCAGCACCTGAATATCTTTCAGGAACAAATCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGTGATATCGGCAGTGACG‑TTCGGGTTGCCGTCGCGGTCGCCGCCCATCCACGAAGTAAAACGGACCGGAACAAATTCGACGGGCAGTTTG > NZ_CP009273/4142159‑4142325 | gTTCAGAAACCAGCACCTGAATATCTTTCAGGAACAAATCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGGGATATTGGGAg > 1:295072/1‑90 (MQ=255) ttCAGAAACCAGCACCTGAATATCTTTCAGGAACAAATCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGGGATATCGGCAgg > 2:103890/1‑89 (MQ=255) gCACCTGAATATCTTTCAGGAACAAATCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGGGATATCGGCAGGGACG‑TTCgggg > 2:123073/1‑89 (MQ=255) tatCTTTCAGGAACAAATCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGTGATATCGGCAGTGACG‑TTCGGGTTGCCGTcgc < 1:314848/90‑1 (MQ=255) tttCAGGAACAAATCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGGGATATCGGCAGGGGCG‑TTTGGGGTGGCGGCGCgggc > 1:351601/1‑88 (MQ=255) tttCAGGAACAAATCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGGGATATCGGCAGGGACG‑GTCGGGGTGGCGTCGCgggc > 2:333718/1‑88 (MQ=255) ttCAGGAACAAATCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGGGATATCGGCAGTGACG‑TTCGGGGTGCCGTCCCGGGcg > 2:143380/1‑90 (MQ=255) tCAGGAACAAATCGGTGGCTTTCCAGCGTCTGAGTAGCAGGACGTGGCGGGTGGGATCGGCAGTGGAG‑TGCCGGGTGCCGTCCCGGGcgg > 1:499752/1‑89 (MQ=255) cAGGAACAAATCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGTGATATCGGCAGTGACG‑TTCGGGTTGCCGTCGCGGTcgcc < 2:379892/90‑1 (MQ=255) cAGGAACAAATCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGGGATATCGGCAGTGACG‑TTTGGGGTGGCGTCGCGGGcgcc > 2:476586/1‑90 (MQ=255) cAGGAACAAATCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGGGATATCGGCAGTGAAG‑ATCGGGTTGCCGTAGGGGTcgcg > 2:189673/1‑89 (MQ=255) aCAAATCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGGGATTTTCGCAGGGACGCTTCGGG‑TGCCGCCGCGGGCGCCGcccc > 1:226104/1‑89 (MQ=255) aCAAATCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGGGATATCGGCAGTGACG‑TTCGGGGTGCCGTCGCGGGCGCCGCCCa > 2:318196/1‑90 (MQ=255) tCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGTGATATCGGCAGTGACG‑TTCGGGTTGCCGTCGCGGTCGCCGCCCATCCAc < 1:506167/90‑1 (MQ=255) tCCAGCGGCTGAGTAGCAGGACGTGGCGGGTGATATCGGCAGTGACG‑TTCGGGTTGCCGTCGCGGTCGCCGCCCATCCACGAAGTAAAAc < 2:295072/90‑1 (MQ=255) gCTGAGTAGCAGGACGTGGCGGGTGATATCGGCAGTGACG‑TTCGGGGTGCCGTCGCGGGCGCCGCCCCTCCACGAAGTAAAACGGGCCgg > 2:499618/1‑90 (MQ=255) cGTGGCGGGTGATATCGGCAGGGACG‑TTCGGGGTGCCGTCGCGGGCGCCGCCCCTCCCCGAAGTAAAACGGGCCGGGACAAATTCGACgg > 2:484937/1‑90 (MQ=255) gCGGGGGATATCGGCAGTGACG‑TTCGGGTTGCCGTCGCGGGCGCCGCCCATCCACGAAGTAAAACGGACCGGAACAAATTCGACGGGCAg > 2:477810/1‑90 (MQ=255) gTGATATCGGCAGTGACG‑TTCGGGGTGCCGTCGCGGTCGCCGCCCATCCCCGAAGTAAAAAGGACCGGAACAAATTCGACGGGCAGGTTg > 2:583624/1‑90 (MQ=255) | GTTCAGAAACCAGCACCTGAATATCTTTCAGGAACAAATCGGTGGCTTTCCAGCGGCTGAGTAGCAGGACGTGGCGGGTGATATCGGCAGTGACG‑TTCGGGTTGCCGTCGCGGTCGCCGCCCATCCACGAAGTAAAACGGACCGGAACAAATTCGACGGGCAGTTTG > NZ_CP009273/4142159‑4142325 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |