| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,225,954 | 0 | A | G | 62.5% | ‑0.1 / 15.4 | 16 | S41G (AGT→GGT) | yjbE | exopolysaccharide production protein YjbE |
| Reads supporting (aligned to +/- strand): ref base A (0/6); new base G (10/0); total (10/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.25e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.53e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGCGGTGATG > NZ_CP009273/4225876‑4226029 | gcggcgACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGGGGTTCTCcgcg > 2:436370/1‑90 (MQ=255) cgACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCa < 1:565861/90‑1 (MQ=255) tGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAg < 1:486410/90‑1 (MQ=255) gggCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGGGTTTCTCCGCGGCCCGCGGCCGCACCg > 2:340499/1‑90 (MQ=255) gggCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGGGGTTCCCCCCGACCAGCGGCAGCAccc > 2:389788/1‑89 (MQ=255) cACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCt < 1:92588/90‑1 (MQ=255) aGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGGGTTCCTCCGCGACCAGCGGCAGCACCGTAAGCTCGGCggggg > 2:484621/1‑90 (MQ=255) ggTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGGGTTTCTCCCCGGCCCGCGTCAGCACCGGAAACTCCGCgggggg > 2:25638/1‑90 (MQ=255) ggTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGGGTTCCTCCCCGACCCGCGGCAGCACCGTAAGCTCGGCgggggg > 2:577806/1‑90 (MQ=255) tGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGGGGTCCTCCCCGGCCCGCGGCCGCCCCCCAAGCCCCGCgggggggg > 1:592596/1‑88 (MQ=255) tGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGGGGTCCTCCCCCACCCGCGGCAGCACCCTAAGGTCGGCgggggggg > 1:437704/1‑88 (MQ=255) gggCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGGGTTCCTCCGCGCCCAGCGGCAACACCGTAAGCTCGGCggggggggg > 2:143463/1‑87 (MQ=255) gggCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGGGGTCCTCCCCGACCCGCGTCAGCACCGTAAACTCGGCggggggggg > 2:293580/1‑87 (MQ=255) ggCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCgg < 1:500636/90‑1 (MQ=255) cGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGCGGTGATg < 1:484621/90‑1 (MQ=255) cGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGCGGTGATg < 1:487543/90‑1 (MQ=255) | GCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGCGGTGATG > NZ_CP009273/4225876‑4226029 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |