| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,245,973 | 0 | T | G | 61.1% | 9.0 / 14.5 | 18 | N141T (AAC→ACC) | plsB | glycerol‑3‑phosphate 1‑O‑acyltransferase PlsB |
| Reads supporting (aligned to +/- strand): ref base T (1/6); new base G (11/0); total (12/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.77e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.86e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGC > NZ_CP009273/4245891‑4246062 | cGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTGACttt > 1:450531/1‑89 (MQ=255) caAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCAc < 1:562025/90‑1 (MQ=255) gACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTTACTTTGCCTTTTTTTCCCCCCCgcgcgc > 1:41031/1‑90 (MQ=255) gACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCCTTTTTACGCCCCCgcgcgc > 2:464593/1‑90 (MQ=255) cGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGAc < 1:233282/90‑1 (MQ=255) aGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCCTTTTTACGCCCCCGCGCGCGACCAAACATc > 2:405933/1‑90 (MQ=255) cAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCg < 1:226350/90‑1 (MQ=255) aGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCTTTTTTTCGCCCCCGCGCGCGCACAAACATCAccca > 2:151452/1‑88 (MQ=255) gCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCTTTTTCCCGCCCCCGGGCGCGACCAAAAATCACCCac > 2:131433/1‑90 (MQ=255) gAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGacac < 2:242967/90‑1 (MQ=255) aaaTTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCTTTTTCACGCCCCGGCGCGGGGCCAAACAACACCGACACTgg > 2:304716/1‑90 (MQ=255) aaaTTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCTTTTTCACGCCCCCGCGCGCGACCAAACATCACCCACACTgg > 2:444884/1‑90 (MQ=255) aTTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCa < 1:361369/90‑1 (MQ=255) gTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCCCCTTTTTTTCCCCCCCGCCCGCGACCCAACAACACCCACCCCGGCACCctct > 1:84094/1‑90 (MQ=255) ttAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATcc < 2:186548/90‑1 (MQ=255) tACGCAGCGGCGGGTTCACTTCGCCTTTTTTACGCCCCCGCGCGCGACCAAAAATCACCCACACTGGCACCCTCTGCACATCCAGAtttt > 2:527697/1‑89 (MQ=255) tACGCAGCGGCGGGGTCACTTCGCCCTTTTTACCCCCCGGCGCGCGGCCCAAAAACACCCCCACCGGCCCCATCTTCACCGCCCGATTTg > 1:488518/1‑90 (MQ=255) cGCAGCGGCGGGGTGACTTCGCCTTTTTTACGCCCCCGCGCGCGGCCCAAAAACACCCACACCGGCCCCCTCTTCCCATCCCGAATTtgg > 1:275808/1‑90 (MQ=255) ttCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGc < 2:409440/90‑1 (MQ=255) | CGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGC > NZ_CP009273/4245891‑4246062 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |