| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,612,067 | 0 | A | G | 54.2% | 9.8 / 27.7 | 24 | E161G (GAG→GGG) | yjjJ | type II toxin‑antitoxin system HipA family toxin YjjJ |
| Reads supporting (aligned to +/- strand): ref base A (3/8); new base G (12/1); total (15/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.23e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.47e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGGAAATTCCTCTGGATCAAAAACTCACCCATTACGAACAGCTGGCAAGT > NZ_CP009273/4611979‑4612155 | gatgatATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCggggag < 2:264176/90‑1 (MQ=255) tctcTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGGTGGTTGGTCGGGGGGGGGGATTATCa > 2:40426/1‑90 (MQ=255) ctctGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGGCGGGGGGGGGGATTATCAg > 2:368046/1‑90 (MQ=255) tGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGa < 2:194417/90‑1 (MQ=255) cAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATgg < 1:451165/90‑1 (MQ=255) aGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGGTGGTCGGGGGGGGGGATTATTAGCGATGGa > 2:232399/1‑90 (MQ=255) agaagaTGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTAc < 1:127541/90‑1 (MQ=255) agaagaTGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTAc < 2:295950/90‑1 (MQ=255) agaagaTGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTAc < 2:154793/90‑1 (MQ=255) agaagaTGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTAc < 1:76771/90‑1 (MQ=255) agaagaTGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTAc < 1:310580/90‑1 (MQ=255) gCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGGTGGTTGGGCGGGGGGGGGGATTATTAGCGATGGAGTACTGGacaaca > 1:282792/1‑90 (MQ=255) tttAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGGGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGGAAATTCCTCTg < 1:32717/90‑1 (MQ=255) gTGAATATACTGGCGGTTGGTTGGGCGGGGGGGGGAATTATCAGCGATGGATTACTGCACAAAACCCCGCGGGAATTCCTCTGGATCaaa > 1:498546/1‑90 (MQ=255) gTGAATATACTGGCGGTTGGGTGGTCGGGGGGGGGGATTATTAGCGATGGATTACTGCACAAAACCCTGCGGGAAGTTCTCTGGGTCaaa > 1:433381/1‑90 (MQ=255) tGGCGGTTGGTTGGGCGGGGGGGGGGATTATCAGCGGTGGGTTACTGCACAACACCCCGCGGGAAATTCTCTGGGTCAAAAAATCACCCa > 1:48799/1‑90 (MQ=255) tGGCGGTTGGTTGGGCGGGGGGGGGGATTATCAGCGATGGATTACTGGACAACACCCCGCGGGAATTTCTCTGGGTTAAAAAATCACCCa > 1:436440/1‑90 (MQ=255) gCGGTTGGGTGGTCGGGGGGGGGAATTATCAGCGATGGATTACTGCACAACACCCCGCGGGAAATCCTCTGGATCAAAAAATCACCCAtt > 1:407311/1‑90 (MQ=255) ggttggttGGTCGGGGGGGGGAAATATCAGCGATGGATTACTGCACAACACCCTGCGGGAAATCCTCTGGATCAAAAACTCACCCATTAc > 1:492973/1‑90 (MQ=255) ggttggttGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGGAAATTCCTCTGGATCAAAAACTCACCCATTAc > 2:461151/1‑90 (MQ=255) ggttggttGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGGAAATTCCTCTGGATCAAAAACTCACCCATTAc > 2:504287/1‑90 (MQ=255) ggttggttGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGGAAATTCCTCTGGATCAAAAACTCACCCATTAc > 1:281481/1‑90 (MQ=255) ggttggttGGGCGGGGGGGGGGATTATCAGCGATGGATTACTGCACAACACCCCGCGGGAAATCCTCTGGATCAAAAACTCACCCATTAc > 1:321685/1‑90 (MQ=255) ggttgggtGGTCGGGGGGGGGGATTTTTAGCGATGGATTACTGCACAAAACCCTGCGGGAATTGCTCTGGATCAAAAAATCAGCCAtttc > 1:136982/1‑88 (MQ=255) tggtCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGGAAATTCCTCTGGATCAAAAACTCACCCATTACGAACAGc < 2:436440/90‑1 (MQ=255) ggggaggggaATTATCAGCGATGGATTACTGCACAACACCCTGCGGAAATTCCTCTGGATCAAAAACTCACCCATTACGAACAGCTGGCa < 1:605630/90‑1 (MQ=255) ggggaggggaATTATCAGCGATGGATTACTGCACAACACCCTGCGGAAATTCCTCTGGATCAAAAACTCACCCATTACGAACAGCTGGCa < 2:564054/90‑1 (MQ=255) gaggggaATTATCAGCGATGGATTACTGCACAACACCCTGCGGAAATTCCTCTGGATCAAAAACTCACCCATTACGAACAGCTGGCAAGt < 1:368046/90‑1 (MQ=255) | GATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGTTGGTTGGTCGGGGAGGGGAATTATCAGCGATGGATTACTGCACAACACCCTGCGGAAATTCCTCTGGATCAAAAACTCACCCATTACGAACAGCTGGCAAGT > NZ_CP009273/4611979‑4612155 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |