| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 5,159 | 0 | G | A | 60.0% | ‑0.9 / 20.3 | 20 | intergenic (+139/‑75) | thrC/yaaX | threonine synthase/DUF2502 domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base G (8/0); new base A (0/12); total (8/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.94e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.77e-05 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAA‑GGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAA > NZ_CP009273/5074‑5239 | tttATGAAGAAATTATGGAGAAAAATGACAGGGAAAAA‑GGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATa > 2:192954/1‑90 (MQ=255) tGAAGAAATTATGGAGAAAAATGACAGGGAAAAA‑GGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATaacaa > 1:65714/1‑90 (MQ=255) gaagaaATTATGGAGAAAAATGACAGGGAAAAA‑GGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATaacaac > 2:111724/1‑90 (MQ=255) agaAAAATGACAGGGAAAAA‑GGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATc > 2:471454/1‑90 (MQ=255) aaaTGACAGGGAAAAA‑GGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGt > 1:512105/1‑90 (MQ=255) agggaaaaagggaaaaTTTCCCAATAAATGGGTTATTTTAGAGATTGGTTTTGGGGAAAATAACACCCGCCGTTCTCATCGAGTAAtctc < 2:237573/90‑1 (MQ=255) aaa‑GGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGatat > 1:320075/1‑90 (MQ=255) agggAAAAATTCTCAAAAATTGTGGTTATTTGAAGATTGGTTTTGGGGAAACCACCAACCGCCGTTCTCATCGAGTAATCTCCGGATATc < 2:33134/88‑1 (MQ=255) tcAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACgg > 1:83391/1‑90 (MQ=255) tcAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACgg > 1:58176/1‑90 (MQ=255) aaTAAACGCGGTAACTAAGAGATTTGGATTGCGGAAAAAAACACCCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGc < 2:166395/90‑1 (MQ=255) tAATTGGGGTAATTTAGATATTGGGTTTGCGGAAAAAAAAACCCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCaa < 1:58688/90‑1 (MQ=255) aaaaTGGGGTAACTAAGAAATTAGGTTGGGGGAAAAAAACACCCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCaa < 2:512105/89‑1 (MQ=255) aaaaTGCGGTATCTTAAAGTTTAGGATGGCGAAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCaa < 2:202002/89‑1 (MQ=255) ggTAATTAAGAGATTAGGTTGGGGGAAAAAAAAAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATaaa < 2:65714/90‑1 (MQ=255) tttAGAGTTTGGGTTGGCGAAAAAAAAAAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAg < 2:527320/89‑1 (MQ=255) tAGAAATTAGGATTGGGGAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTa < 2:320075/90‑1 (MQ=255) aagagATTAGGATGGGGAAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTa < 2:123398/89‑1 (MQ=255) tAGGATGGGGGAAAAAAACACCCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCtgtg < 1:160255/90‑1 (MQ=255) gATTGGGGAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGaaa < 2:97920/90‑1 (MQ=255) | TTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAA‑GGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAA > NZ_CP009273/5074‑5239 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |