Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	49,903	T→G	D27E (GAT→GAG)	folA →	type 3 dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	49,903	0	T	G	100.0%	50.8 / NA	17	D27E (GAT→GAG)	folA	type 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (7/10);  total (7/10)

CAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTG  >  NZ_CP009273/49828‑49984                                                                            |                                                                                  cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTaa                                                                     >  1:80521/1‑90 (MQ=255)  aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACCCCATGGCGGGGAACCTGGCTTCCGAGCTCGCCTGGTTTaaa                                                                    >  2:398990/1‑90 (MQ=255)       gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCaa                                                               <  2:571889/90‑1 (MQ=255)       gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCaa                                                               <  2:380580/90‑1 (MQ=255)       caTTGCGGCCTAAGCGGTAGATCGCGTTACCGGCATGGAAAACGCAATGCCGTGTAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCaa                                                               <  1:398990/89‑1 (MQ=255)        aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCaa                                                               >  1:595042/1‑89 (MQ=255)        aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCaa                                                               <  2:595042/89‑1 (MQ=255)                        tAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCg                                              <  1:583286/90‑1 (MQ=255)                          gATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTg                                            >  2:491911/1‑90 (MQ=255)                                 ttATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg                                     <  2:9900/90‑1 (MQ=255)                                 ttATCGCCATGGAAAACGCCATGCCGGGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg                                     <  2:418316/90‑1 (MQ=255)                                            gAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACCCCTTAAATAACCCCGTGATTATGGGCCGCCATAcc                          <  2:490349/90‑1 (MQ=255)                                                 cGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGa                     <  1:61521/90‑1 (MQ=255)                                                                 ctgcctgcCGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCg     >  2:381167/1‑90 (MQ=255)                                                                    cctgccGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTg  >  2:270523/1‑90 (MQ=255)                                                                    cctgccGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTg  >  2:89396/1‑90 (MQ=255)                                                                         cGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATggg                                    <  2:447248/51‑1 (MQ=255)                                                                            |                                                                                  CAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTG  >  NZ_CP009273/49828‑49984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑