| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 899,438 | 0 | A | C | 57.1% | 11.0 / 15.5 | 14 | G162G (GGT→GGG) | ybjP | lipoprotein |
| Reads supporting (aligned to +/- strand): ref base A (6/0); new base C (0/8); total (6/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATGCCGGACAATTTACGGGGTTTATTGGTTGATCAAGGCGTTAGCGATTCTCGATGGACTGACGGAGCGTACCCGCCGTGGCATGAACGCTACCGCCTAAGTAACGCACATCGTCGATGACCCAACACTGGCCTTCCTGAATCATTAACACTTCATCCTG > NZ_CP009273/899368‑899527 | aTGCCGGACAATTTACGGGGTTTATTGGTTGATCAAGGCGTTAGCGATTCTCGATGGACTGACGGAGCGTACCCGCCGTGGCATGAACGc > 2:433066/1‑90 (MQ=255) gCCGGACATTTTGGGGGGTTTTTTGGTTGATCAGGGCGTTAGCGTTTCTCGATGGACTGACGGAGCGTCCCCGCCGTGGCATGAACGCTa < 1:597020/90‑1 (MQ=255) aaaaTTTAGGGGTTTTATTGGTTGAACAGGGCGTTGGCGTTTCTGGAGGGACGGAGGGAGCGTCCCCGCCGTGGCATGAACGCTACCGcc < 1:433066/88‑1 (MQ=255) gTTTATTGGTTGATCAAGGCGTTAGCGATTCTCGATGGACTGACGGAGCGTACCCGCCGTGGCATGAACGCTACCGCCTAAGTAACGcac > 2:871283/1‑90 (MQ=255) ttATGGGTTGACCAGGGCGTTAGCGTTTCCCGATGGACTGAGGGAGCGTCCCCGCCGTGGCATGAACGCTACCGCCTAAGTAACGCACAt < 2:772619/90‑1 (MQ=255) ttGATCAGGGGGTTAGCGTTTCTCGATGGACTGACGGAGCGTCCCCGCCGTGGCATGAACGCTACCGCCTAAGTAACGCACATCGTCGAt < 2:904009/90‑1 (MQ=255) ttGATCAAGGCGTTAGCGATTCCCGATGGACTGAGGGAGCGTCCCCGCCGTGGCATGAACGCTACCGCCTAAGTAACGCACATCGTCGAt < 2:339835/90‑1 (MQ=255) ttGATAAGGGCGTTAGCGATTCTCGAGGGCCGGGCGGAGCGTCCCCGCCGTGGCATGAACGCTACCGCCTAAGTAACGCACATCGTCGAt < 2:742239/90‑1 (MQ=255) ttGATAAAGGCGTTAGCGATTCTCGATGGACTGACGGAGCGTCCCCGCCGTGGCATGAACGCTACCGCCTAAGTAACGCACATCGTCGAt < 2:4201/90‑1 (MQ=255) tAGCGATTCTCGATGGACTGACGGAGCGTACGCGCCGTGGCATGAACGCTACCGCCTAAGTAACGCACATCGTCGATGACCCAACACTgg > 2:730253/1‑90 (MQ=255) tAGCGATTCTCGATGGACTGACGGAGCGTACCCGCCGTGGCATGAACGCTACCGCCTAAGTAACGCACATCGTCGATGACCCAACACTgg > 1:206636/1‑90 (MQ=255) tAGCGATTCTCGATGGACTGACGGAGCGTACCCGCCGTGGCATGAACGCTACCGCCTAAGTAACGCACATCGTCGATGACCCAACACTgg > 1:823648/1‑90 (MQ=255) tctcGATGGACTGACGGAGCGTACCCGCCGTGGCATGAACGCTACCGCCTAAGTAACGCACATCGTCGATGACCCAACACTGGCCTTCCt > 2:487797/1‑90 (MQ=255) ctcGATGGACGGAGGGAGCTTCCCCGCCGTGGCATGAACGCTACCGCCTAAGTAACGCACATCGTCGATGACCCAACACTGGCCTTCCTg < 1:302411/90‑1 (MQ=255) aCCCGCCGTGGCATGAACGCTACCGCCTAAGTAACGCACATCGTCGATGACCCAACACTGGCCTTCCTGAATCATTAACACTTCATCCTg > 1:345915/1‑90 (MQ=255) | ATGCCGGACAATTTACGGGGTTTATTGGTTGATCAAGGCGTTAGCGATTCTCGATGGACTGACGGAGCGTACCCGCCGTGGCATGAACGCTACCGCCTAAGTAACGCACATCGTCGATGACCCAACACTGGCCTTCCTGAATCATTAACACTTCATCCTG > NZ_CP009273/899368‑899527 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |