| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 976,689 | 0 | A | G | 62.5% | ‑2.5 / 12.8 | 16 | I63V (ATC→GTC) | ldtD | L,D‑transpeptidase |
| Reads supporting (aligned to +/- strand): ref base A (1/5); new base G (10/0); total (11/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.37e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.47e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCCCTGGCGACAGCCCGGTGGCTGTCAGTGAACAGGGCGAGGCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGATCCAGCCATTGCCTGAAGGTGCGGCAGAAAAAGCCCGCACGCAAATCGAATCTCAATTACCCGCAGGTTACAAGCCGGTTTATCTTAAC > NZ_CP009273/976609‑976778 | tCCCTGGCGACAGCCCGGTGGCTGTCAGTGAACAGGGCGAGGCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGATCCAGCCAt < 2:682387/90‑1 (MQ=255) cTGGCGACAGCCCGGTGGCTGTCAGTGAACAGGGCGAGGCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGGGCCAGCCCTTGc > 1:65262/1‑90 (MQ=255) tGGCGACAGCCCGGTGGCTGTCAGTGAACAGGGCGAGGCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGGTCCAGCCATTGcc > 2:245121/1‑90 (MQ=255) gACAGCCCGGTGGCTGTCAGTGAACAGGGCGAGGCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGGGCCCGCCCCTTCCTGaa > 2:646884/1‑90 (MQ=255) tGTCAGTGAACAGGGCGAGGCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGGTCCAGCCCTTGCCTGAAGGGGGGGCAGaaaa > 2:59154/1‑90 (MQ=255) tGTCAGTGAACAGGGCGAGGCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGGTACAGCCACTGCCCGAAAGGGCGGGAGgaaa > 2:596883/1‑90 (MQ=255) tGTCAGTGAACAGGGCGAGGCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGGGCCAGCCATTTCCTCAAGGGGGGGGGGaaaa > 2:811677/1‑90 (MQ=255) aGGGCGAGGCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGATCCAGCCATTGCCTGAAGGTGCGGCAGAAAAAGCCcgcacgc < 2:883625/90‑1 (MQ=255) gggCGAGGCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGGTCCAGCCCTTGGCTGAAGGGGCGGGAGAAAAAACCcgcacgca > 2:18281/1‑90 (MQ=255) gAGGCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGATCCAGCCATTGCCTGAAGGTGCGGCAGAAAAAGCCCGCACGCAAATc < 2:846283/90‑1 (MQ=255) aGGCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGGTCCAGCCATTGCCTGAAGGGGCGGCAGAAAAAACCCCCACGCCAAACg > 2:272055/1‑90 (MQ=255) cTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGGGCCCGCCCCTGCCCGAAAGGGCGGCGGGAAAAAACCCCCCGCCAAACGAAact > 1:556652/1‑90 (MQ=255) cTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGATCCAGCCATTGCCTGAAGGGGCGGCGGAAAAAACCCGCACGCAAATCGAAtct > 2:178087/1‑90 (MQ=255) aCGGCAATAATGGCGGGGGGCCAGCCCTTGCCCGAAAGGGGGGGCGAAAAAAACCCCCCGCCAAACGAAACTCAATTAACCCCACGGTAc > 1:538227/1‑90 (MQ=255) cAATAATGGCGGGGATCCAGCCATTGCCTGAAGGTGCGGCAGAAAAAGCCCGCACGCAAATCGAATCTCAATTACCCGCAGGTTACAAGc < 2:394284/90‑1 (MQ=255) atGGCGGGGATCCAGCCATTGCCTGAAGGTGCGGCAGAAAAAGCCCGCACGCAAATCGAATCTCAATTACCCGCAGGTTACAAGCCGGtt < 2:412617/90‑1 (MQ=255) aTCCAGCCATTGCCTGAAGGTGCGGCAGAAAAAGCCCGCACGCAAATCGAATCTCAATTACCCGCAGGTTACAAGCCGGTTTATCTTAAc < 1:18281/90‑1 (MQ=255) | TCCCTGGCGACAGCCCGGTGGCTGTCAGTGAACAGGGCGAGGCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGATCCAGCCATTGCCTGAAGGTGCGGCAGAAAAAGCCCGCACGCAAATCGAATCTCAATTACCCGCAGGTTACAAGCCGGTTTATCTTAAC > NZ_CP009273/976609‑976778 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |