| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 984,360 | 0 | T | G | 52.6% | 21.8 / 25.1 | 19 | T28P (ACC→CCC) | asnS | asparagine‑‑tRNA ligase |
| Reads supporting (aligned to +/- strand): ref base T (1/8); new base G (10/0); total (11/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.19e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.61e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTACAGGATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCCCTGGAGTACGTCGGCTACAGGCACAACGCTCATA > NZ_CP009273/984291‑984442 | gTACAGGATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGGACGTACCCCTCCCCGCACgg > 2:14234/1‑90 (MQ=255) aGGATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGGACGCAACCATGCACCCACgggggc > 1:447757/1‑86 (MQ=255) gATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGGACGGACCCATCCGCGCCCCGGGACtt > 2:676137/1‑90 (MQ=255) cAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGc < 1:854550/90‑1 (MQ=255) aaaGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGGACGTACCCATCCCCGCACGGGGGCTTTGCt > 2:773017/1‑90 (MQ=255) aaaGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGGACGTACCCATCCCCGCACGGGGACTTCGCt > 1:155188/1‑90 (MQ=255) aaaGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGGACGGACCCATCCCCGCACGGGGACTTCGCt > 1:368105/1‑90 (MQ=255) aaaGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGGACCGACCCCCCCCCCCACGGGGGCCTCGCt > 1:622396/1‑90 (MQ=255) ggAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGGACGGACCCAACCGCCCACGGGGACTTCGCCGTCAAc > 2:864685/1‑90 (MQ=255) cGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACCGACCCCCCCCCGCCCGGGGGCTTATCTGTCAAAGGcga > 1:726780/1‑88 (MQ=255) gTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGGACGGACCCCTCCGCGCACGGGGACTTCGCTGTCAACGGCTac > 2:901003/1‑90 (MQ=255) cATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTacac < 2:169494/90‑1 (MQ=255) cGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCCCt < 1:68294/90‑1 (MQ=255) gCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGGACGTACCCCTCCCCGCACGGGGACTTCGCTGTCAACGGGCACACCGCCCCgg > 1:266994/1‑90 (MQ=255) tttGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCCCTGGAGTACGTCGGCTACAGGcaca < 1:901003/90‑1 (MQ=255) tGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCCCTGGAGTACGTCGGCTACAGGCACAAc < 2:447757/90‑1 (MQ=255) gAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCCCTGGAGTACGTCGGCTACAGGCACAACg < 2:89324/90‑1 (MQ=255) aTCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCCCTGGAGTACGTCGGCTACAGGCACAACGCt < 1:951373/90‑1 (MQ=255) cggcggGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCCCTGGAGTACGTCGGCTACAGGCACAACGTTCata < 1:31177/90‑1 (MQ=255) | GTACAGGATCAAAGCAGGAACCGTCATAAACGGCGAGGAAGGAGATGCCAGCTTTTGAATCTCGGCGGGTACGTACCCATCCGCGCACGGTGACTTCGCTGTCAACGGCTACACGGCCCTGGAGTACGTCGGCTACAGGCACAACGCTCATA > NZ_CP009273/984291‑984442 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |