| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,377,991 | 0 | A | C | 58.3% | 8.4 / 11.3 | 12 | G76G (GGT→GGG) | ycjW | LacI family DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (5/0); new base C (0/7); total (5/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.26e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCT > NZ_CP009273/1377907‑1378070 | gtgCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACccgc > 2:510125/1‑90 (MQ=255) tGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTg > 1:488543/1‑90 (MQ=255) tCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAAc > 2:935594/1‑90 (MQ=255) cACGCCAAACCCTTCTTCTTCATTTTTGGGGGTGAGGGCATGAAAAACTTAAGAAAAAAACGGATTCCCCGCCGTAGTTTTGGTCGAACg < 1:118111/90‑1 (MQ=255) aCGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACgg > 2:219688/1‑90 (MQ=255) gTCAAACCCTTTTTCTTCAGCTTGGGCGGTGATGGCATGAAAACCTTAAGAAAAAAACGGATTCCCCGCCGTAGTTTTGGTCGAACGGGt < 2:633451/90‑1 (MQ=255) tCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTa > 1:775737/1‑90 (MQ=255) aaCCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAAAAAAACGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGaaa < 2:323990/90‑1 (MQ=255) tcttcttcAGCTTTGGCGGTGGTGGCATAAAAACCTTAAAAAAAAACCGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCAcc < 1:777765/90‑1 (MQ=255) ggggATGGCATAAAAACCTTAAAAAAAAAACGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAAcc < 1:801337/87‑1 (MQ=255) cAAAACTTAAGAAAAAACCGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAg < 2:488543/90‑1 (MQ=255) aaaaCGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCt < 2:624135/90‑1 (MQ=255) | GTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCT > NZ_CP009273/1377907‑1378070 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |