| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,380,342 | 0 | C | A | 56.2% | 8.6 / 20.3 | 16 | intergenic (+32/‑71) | yfbL/yfbM | M28 family metallopeptidase/YfbM family protein |
| Reads supporting (aligned to +/- strand): ref base C (1/6); new base A (9/0); total (10/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.74e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.07e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGA > NZ_CP009273/2380254‑2380405 | taGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCg < 2:18922/89‑1 (MQ=255) cAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACattt < 1:679233/90‑1 (MQ=255) gTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAAAGGCAATCACattttttttt > 2:679233/1‑90 (MQ=255) gTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAAAGAAAAACACaatttttttt > 1:117398/1‑90 (MQ=255) tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATc < 2:238839/90‑1 (MQ=255) tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATc < 2:300419/90‑1 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAAAGACAAGCACATTTTTTTTTTCCTCCTCTGTTTTTAg > 2:250728/1‑90 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGTCCGAAAAAAAGACAAGCAAATTTTTTTTTTTCTCCTCTGtttttaa > 1:264366/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAGACATGCACATTTTTTTTTTTCTCCTCTGtttttaa > 2:532133/1‑87 (MQ=255) aGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAACGACATGCACATTTTTTTTTTCCTCATCTGTTTTTAGATGGGGAACATGa > 2:303819/1‑90 (MQ=255) aaaTAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAGACAAGCACATTTTTTTTTTCCTCCTCTTTTTTTAGAAGCGAAAAATaaaaa > 2:948499/1‑89 (MQ=255) taataaAAATTTCTCGAGGCGGGGCCCGAAAAAAAGAAAAGCACAATTTTTTTTTTCTCATCTTTTTTTAAATGGGAAAAAAGaaaatta > 1:945066/1‑86 (MQ=255) ataaAAATTTCTCGAGGCGGGTCCCGAAAAAAAGACAAGCACATTTTTTTTTTCCCCATCTGTTTTTAAATGCGGAAAAAGAAAATCAAc > 2:63640/1‑90 (MQ=255) ataaAAATTTCTCGAGGCGGGGGCCGAAAAAAAGACATGGACAATTTTTTTTTTCTCCTCTTTTTTTAAATGCGGAACAAGAAAATCAAc > 1:410108/1‑90 (MQ=255) aaaaTTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACgga < 1:349504/90‑1 (MQ=255) aTTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACggagga < 2:572217/90‑1 (MQ=255) | CAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGA > NZ_CP009273/2380254‑2380405 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |