Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,341,2370TG54.2% 21.5 / 25.2 24V255G (GTG→GGG) rapZRNase adapter RapZ
Reads supporting (aligned to +/- strand):  ref base T (5/6);  new base G (13/0);  total (18/6)
Fisher's exact test for biased strand distribution p-value = 3.43e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.66e-04
Rejected as consensus: Frequency below/above cutoff threshold.

CTTGAGCTATGGTTACCTATGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAA  >  NZ_CP009273/3341149‑3341321
                                                                                        |                                                                                    
cTTGAGCTATGGTTACCTATGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGgtg                                                                                     <  2:68725/90‑1 (MQ=255)
        aTGGTTACCTATGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGGGTATATTGc                                                                             >  2:94357/1‑90 (MQ=255)
        aTGGTTACCTATGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGGGGATAtttc                                                                             >  2:283782/1‑88 (MQ=255)
        aTGGTTACCTATGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCCCTCGGTGTGTATTGc                                                                             >  1:667502/1‑90 (MQ=255)
             tACCTATGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGGAGGACCCCTCGGGGGGTATTTCAGAGc                                                                        >  2:472941/1‑90 (MQ=255)
                 tATGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCATAGCAACt                                                                    <  1:378039/90‑1 (MQ=255)
                  aTGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGGAGGACCCTTTGGGGTATATTGCAGAGCAACTg                                                                   >  2:590491/1‑90 (MQ=255)
                  aTGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCCTTCGGTGGATATTGCCGAGCAACTg                                                                   >  1:931005/1‑90 (MQ=255)
                              aacaacCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGGGTATATTGCAGAGGAAATGGCCGACTACCTc                                                       >  2:175978/1‑90 (MQ=255)
                               acaacCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGgtg                                                                                     >  2:129729/1‑59 (MQ=255)
                               acaacCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGgtg                                                                                     <  1:129729/59‑1 (MQ=255)
                                      tAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGGAGCACCGTTCGGGGGATATTGCAGAGCAACTGGGAGACTACTTCCGCTcgcg                                               >  2:431488/1‑90 (MQ=255)
                                      tAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGGAGCACCCTTTGGGGGATATTTCAGAGGAACTGGGGGACTACTTTCGCTcccg                                               >  2:573074/1‑90 (MQ=255)
                                      tAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGGTCGGGGGATATTGCAGAGCAAATGGCCGACTACTTCCCCCcgcg                                               >  1:893589/1‑90 (MQ=255)
                                            cTTGACGGTCGCCATTGGTTGTACCGGCGGGGAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCCGGCTACTTCCCCCCCCGCGGGaa                                         >  1:858692/1‑90 (MQ=255)
                                             ttGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGGGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGGaaa                                        >  2:46480/1‑90 (MQ=255)
                                                   gTCGCCATTGGTTGTACCGGCGGGGAGCACCGTTCGGGGTATATTGCAGAGGAACTGGCAGACTACTTCCCCTCCCGCGGGaaaaaaatc                                  >  2:861501/1‑86 (MQ=255)
                                                     cGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGGGTATATTGCAGAGCAACTGGCAGACTACTTCCCCTCCCGCGGGAAAAAAGTccc                                >  2:769038/1‑89 (MQ=255)
                                                          ttGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCAc                           <  2:60103/90‑1 (MQ=255)
                                                            ggTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGc                         <  1:278461/90‑1 (MQ=255)
                                                            ggTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGc                         <  1:730718/90‑1 (MQ=255)
                                                               tGTACCGGCGGGAAGGACCCCTCGGGGTATATTGCAGAGCAAATTGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCAt                      >  2:808242/1‑90 (MQ=255)
                                                                      gcggGAAGCACCGTTCGGGGGATATAGCAGAGCAACTGGCAGACTACTTCCGCCCCCGCGGGAAAAAAGTCCAGTCACCCCCTCGTACGc               >  1:330898/1‑90 (MQ=255)
                                                                            aGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGaaa         <  2:98796/90‑1 (MQ=255)
                                                                                  gTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTa   >  2:336710/1‑90 (MQ=255)
                                                                                  gTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTa   >  1:563834/1‑90 (MQ=255)
                                                                                   ttCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTaa  <  1:938247/90‑1 (MQ=255)
                                                                                        |                                                                                    
CTTGAGCTATGGTTACCTATGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAA  >  NZ_CP009273/3341149‑3341321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑