Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 49,910 T→G W30G (TGG→GGG)  folA → type 3 dihydrofolate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP00927349,9100TG97.4% 138.3 / ‑3.5 39W30G (TGG→GGG) folAtype 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (14/24);  total (14/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.23e-01

CAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAA  >  NZ_CP009273/49828‑49996
                                                                                  |                                                                                      
cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTaa                                                                                 >  2:150924/1‑90 (MQ=255)
 aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTaaa                                                                                >  2:952014/1‑90 (MQ=255)
  gTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAAc                                                                               >  1:412841/1‑90 (MQ=255)
   tCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGTAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACg                                                                              >  2:840169/1‑90 (MQ=255)
      gtttGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGACCCTGCCTGCCGATCTCGCCGGGTTTAAACGCaa                                                                           <  1:886727/88‑1 (MQ=255)
      gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCaa                                                                           <  2:379124/90‑1 (MQ=255)
      gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCaa                                                                           <  1:928429/90‑1 (MQ=255)
       ctgccggcgTTAGCGGTGGCGCGCGTTAGCGGCTTGAAAAACGCCATGCCGTGGAGCCTGCCTGCCGATCTCGCCGGGTTTAAACGCAac                                                                          <  1:382178/86‑1 (MQ=255)
       aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAac                                                                          <  2:203551/90‑1 (MQ=255)
       aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAac                                                                          <  2:621964/90‑1 (MQ=255)
           cggcggTAGCGGTAGATCGCGTTTTCGGCATGGAAAACGCCATGCCGTGGACCCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCt                                                                      <  1:130494/90‑1 (MQ=255)
           cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCt                                                                      <  1:952014/90‑1 (MQ=255)
            ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCtt                                                                     >  1:298097/1‑90 (MQ=255)
                ttAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTtaaat                                                                 >  2:658013/1‑90 (MQ=255)
                      gTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAAccc                                                           <  2:131622/90‑1 (MQ=255)
                       tAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCg                                                          <  2:136994/90‑1 (MQ=255)
                        aGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGt                                                         <  2:51880/90‑1 (MQ=255)
                             gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTa                                                    >  2:274648/1‑90 (MQ=255)
                                ttATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg                                                 >  1:70344/1‑90 (MQ=255)
                                 tATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATggg                                                <  2:70344/90‑1 (MQ=255)
                                     ggCAGGAAAACCGCCCTGCCGTGGACCCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccgc                                            <  1:115507/90‑1 (MQ=255)
                                       cATGAAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCa                                          <  2:234051/90‑1 (MQ=255)
                                        aTGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt                                         <  2:35866/90‑1 (MQ=255)
                                        aTGAAAAACGCCATGCCGTGGACCCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt                                         <  1:925342/90‑1 (MQ=255)
                                                    aTGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAac                                                                          <  2:934014/45‑1 (MQ=255)
                                                    aTGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAac                                                                          >  1:934014/1‑45 (MQ=255)
                                                    aTGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatca                             >  2:829408/1‑90 (MQ=255)
                                                    aTGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGa                                 <  2:276089/86‑1 (MQ=255)
                                                    aTGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGa                                 >  1:276089/1‑86 (MQ=255)
                                                       ccGTGGACCCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatcaatc                          <  1:273630/90‑1 (MQ=255)
                                                           ggAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgtc                      <  1:198379/90‑1 (MQ=255)
                                                                ctccctgccGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCg                 <  2:740271/87‑1 (MQ=255)
                                                                 tgcctgccGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGt                >  2:191180/1‑90 (MQ=255)
                                                                 tgcctgccGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGt                <  2:701403/90‑1 (MQ=255)
                                                                  gcctgccGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatcaatc                          >  1:99924/1‑79 (MQ=255)
                                                                  gcctgccGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatcaatc                          <  2:99924/79‑1 (MQ=255)
                                                                      gccGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCa           >  1:138277/1‑90 (MQ=255)
                                                                            ctcGCCGGGTTTAAACCCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGc     <  1:107772/90‑1 (MQ=255)
                                                                               gCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCaaa  <  2:356887/90‑1 (MQ=255)
                                                                                  |                                                                                      
CAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAA  >  NZ_CP009273/49828‑49996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: