| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,012,931 | 0 | A | C | 54.5% | 0.5 / 10.9 | 11 | T138P (ACA→CCA) | ssnA | putative aminohydrolase SsnA |
| Reads supporting (aligned to +/- strand): ref base A (0/5); new base C (5/1); total (5/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.52e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCGCTCTCCACATTGCGCGACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAACTACTGACCGTAACAACGGCATCAAAG > NZ_CP009273/3012853‑3013012 | cTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGGCGCTCTCCCCCTTTcgcggc > 2:20040/1‑88 (MQ=255) aCATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGGCGCTCTCCCCCTTTCGCGGCGCCTTTTTTAAAAATTGG‑CTgcgc > 2:264268/1‑90 (MQ=255) ggTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGGCGGTCTCCCCCCTTCGCGGCGCCTTTTTTTAAATTTGGCc‑gcgcgcggt > 1:93001/1‑88 (MQ=255) gTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCGCTCTCCACATTGCGCGACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATg < 1:264268/90‑1 (MQ=255) cACCATGCCTCTCCGGCGTATATCGGCGGGGCGCTCCCCCCCCTTCGCGACG‑CCTTTTTTAAAATTGGGCCGCGCGCGGTGGCCTGgttt > 1:35055/1‑90 (MQ=255) tGCCTCTCCGGCGTATATCGGCGGGTCGCTCTCCACATTGCGCGACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAac < 2:121952/90‑1 (MQ=255) ctcCGGCGTATATCGGCGGGTCGCTCTCCCCATTGCGCGACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAACTACTg < 1:164800/90‑1 (MQ=255) ctcCGGCGTATATCGGCGGGTCGCTCTCCACATTGCGCGACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAACTACTg < 2:236628/90‑1 (MQ=255) ctcCGGCGTATATCGGCGGGGCGCCCTCCCCATTTCGCGGCG‑CATTTTTTAAAATTGGCCCGCGCGCGGTGACCCGTTTTTTAaatactt > 2:125046/1‑89 (MQ=255) cGGCGTATATCGGCGGGTCGCTCTCCACATTGCGCGACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAACTACTGAcc < 1:17305/90‑1 (MQ=255) cGCTCTCCACATTGCGCGACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAACTACTGACCGTAACAACGGCATCAAag < 2:93001/90‑1 (MQ=255) | CTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCGCTCTCCACATTGCGCGACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAACTACTGACCGTAACAACGGCATCAAAG > NZ_CP009273/3012853‑3013012 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |