Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,368,6020GT54.5% 6.1 / 16.5 11P54T (CCT→ACT) ampCBlaEC family class C beta‑lactamase
Reads supporting (aligned to +/- strand):  ref base G (5/0);  new base T (0/6);  total (5/6)
Fisher's exact test for biased strand distribution p-value = 2.16e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCAC  >  NZ_CP009273/4368515‑4368680
                                                                                       |                                                                              
tGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGtt                                                                              >  1:62285/1‑90 (MQ=255)
      ccGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCt                                                                        >  2:197326/1‑90 (MQ=255)
                          gTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTaccgccaccgcc                                                    >  1:269309/1‑90 (MQ=255)
                                        gggCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCtt                                      >  1:94308/1‑90 (MQ=255)
                                              ttttttGGCTATGCCCGCACCCCCCCGGGAAAAGAAATAAGTTTTCCCCTAAAAAATTCCCCCCCCCGCCATACCCGGGATCTTttgttg                                <  2:191716/89‑1 (MQ=255)
                                               ttttgGGCGATGCCCGCATACCCCCAGAAAAAAAAATAAGTTTTCCCTTAAAAAATCCCCCCCACCGCCATACCCGGGATCTTTTGTTGc                               <  2:271887/90‑1 (MQ=255)
                                                       gATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAg                       >  1:41733/1‑90 (MQ=255)
                                                                  cgCCCCAGGAAAGGTAATAAGTTTTCCCTTAAAAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATtg            <  2:41733/89‑1 (MQ=255)
                                                                     cccAGGTAAAGTAATAAGTTTTCCCCTGAAAAATTCCCGCCACCGCCATACGCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGc         <  1:112970/90‑1 (MQ=255)
                                                                       cAGGAAAAGAAAAAAGTTTTCCCCTAAAAATTTCCCCCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGa       <  2:94029/90‑1 (MQ=255)
                                                                            aaaGTAATAAGTTTTCCCCTGATAAATTCCCGCCCCCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGcac  <  2:6564/90‑1 (MQ=255)
                                                                                       |                                                                              
TGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCAC  >  NZ_CP009273/4368515‑4368680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑