| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 353,586 | 0 | A | T | 58.8% | ‑1.8 / 17.6 | 17 | N272K (AAT→AAA) | cynR | transcriptional regulator CynR |
| Reads supporting (aligned to +/- strand): ref base A (5/2); new base T (0/10); total (5/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.39e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.85e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGT > NZ_CP009273/353497‑353661 | aGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTC‑GCGGCGGCTGTCTGCCAGCTa > 2:491533/1‑90 (MQ=255) cTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTC‑GCGGCGGCTGTCTGCCAGCTAttttt > 1:741437/1‑90 (MQ=255) gTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTccgcc < 2:744188/90‑1 (MQ=255) gTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTccgcc > 2:633191/1‑90 (MQ=255) cacccGCGCTTTTACCCAACCCCATGTAAAAAAATCCCTCC‑GGGGGGGCTGTCTGCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTtc < 2:331428/87‑1 (MQ=255) ccGCGCTTTTACCCACCCCCATGTCAAAAAATGCCTTC‑GCGGCGGCTGTCTGCCAGTTTTTTTCCCGCCGCAACAAAACCGCCGTtctct < 2:408855/90‑1 (MQ=25) cATTTATCCAACGCCATGTGCAAAAATGCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCagt > 2:430078/1‑90 (MQ=255) aCGCCATGTGCAAAAATGCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgggg > 2:84768/1‑90 (MQ=255) aTGTGCAAAAATGCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTccgcc < 1:787865/49‑1 (MQ=255) aTGTGCAAAAATGCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTccgcc > 2:787865/1‑49 (MQ=255) aaaaaTCCCTCG‑GGGGGGTCTGTCTGCCTTTTTTTTTCCCCCCGCAACAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATa < 1:113792/90‑1 (MQ=255) aaaaTTCCTTCGGGGGGGGTTGTCGGCCACTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATa < 2:399628/90‑1 (MQ=255) ccTCC‑GCGGCGGCTGTCTCCCACTTTTTTTCCCGCCGAAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTaa < 2:413731/90‑1 (MQ=255) tC‑GGGGCGCCTGTCTCCCACCTTTTTTCCCCCCCCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGcc < 2:412808/90‑1 (MQ=255) cc‑gcgGGGTCTCTCTGCCAGTTTTTTTCCCGCCAAAAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGcc < 1:235487/89‑1 (MQ=255) cgggggcggcTCTCGGCCGTTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGcc < 1:1520/86‑1 (MQ=255) ggcgccTGCCTGCCATTTTTTTTCCCCCCGAAAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTc < 1:412330/90‑1 (MQ=255) gcTTTCTGCCAGTTTTTTTCCCCCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCAtgt < 1:633191/90‑1 (MQ=255) | AGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGT > NZ_CP009273/353497‑353661 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |