| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,125,830 | 0 | C | T | 53.3% | 2.6 / 17.4 | 15 | A164T (GCG→ACG) | flgA | flagellar basal body P‑ring formation chaperone FlgA |
| Reads supporting (aligned to +/- strand): ref base C (7/0); new base T (0/8); total (7/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.55e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.72e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGCCTGACCTTCTGCGTTGGCGCTAAACCCATCACCGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTACCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGATAGATCGCGCAGGCTAATGGCATCAAC > NZ_CP009273/1125764‑1125911 | gcgcCTGACCTTCTGCGTTGGCGCTAAACCCATCACCGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTACCCGCCATGCCTGGCGaa > 1:606167/1‑90 (MQ=255)gcgcCTGACCTTCTGCGTTGGCGCTAAACCCATCACCGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTACCCGCCATGCCTGGCGaa > 2:195980/1‑90 (MQ=255) cgcCTGCCCTTCTGCGTTGGCGCAAACCCCACCCCCGCGGGCGATCACTTTGACGCGTTGTCCCGTTTTTCCCCGCCATGCCTGGCGaaa < 1:833931/90‑1 (MQ=255) tCTGCGTTGGCGCTAAACCCCCCCCCCCGGGCAATAACTTGGGGGCGTTTCCCCCTTTTTCCCCGCCATGCCTGGCGAAACTGGGTTAAc < 1:375833/90‑1 (MQ=255) cTGCGTTGGCGCAAACCCCATCCCCGCTGGCGATCACATTGACGCGTTGTCCCGTTTTTACCCGCCATGCCTGGCGAAACTGGGTTAACt < 2:606167/90‑1 (MQ=255) tGCGTTGACGCTAAACCCATCACCGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTACCCGCCATGCCTGGCGAAACTGGGTTAACTg > 1:351813/1‑90 (MQ=255) tGGCGCTAAACCCATCACCGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTACCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAg > 2:197884/1‑90 (MQ=255) cagccccACCCCCGCTGGCAATCACATTGACGCGTTGCCCCGTTTTTACCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGAt < 2:96716/86‑1 (MQ=255) accccACCCCCGCGGGCAATCACATTGCCGGTTTGCCCCTTTTTTCCCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCg < 1:525061/88‑1 (MQ=255) aaCCCACCCCCGCGGGGGATCACATTGACGGTTTGTCCCGTTTTTCCCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCg < 1:716506/90‑1 (MQ=255) gCTGGCGATCACATTGACGCGTTGTCCCGCTTTTACCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGATAgctg > 1:96716/1‑87 (MQ=255) gCTGGCGATCACATTGACGCGTTGTCCCGCTTTTACCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGATAGATc > 2:472664/1‑90 (MQ=255) cGATCACATTGACGCTTTGTCCCTTTTTTCCCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGATAGATCGCGCa < 2:373509/90‑1 (MQ=255) gACGCGTTGCCCCTTTTTCCCCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGATAGATCGCGCAGGCTAATGGc < 1:472542/90‑1 (MQ=255) ttGTCCCGCTTTTACCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGATAGATCGCGCAGGCTAATGGCATcaac > 2:38829/1‑90 (MQ=255) | GCGCCTGACCTTCTGCGTTGGCGCTAAACCCATCACCGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTACCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGATAGATCGCGCAGGCTAATGGCATCAAC > NZ_CP009273/1125764‑1125911 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |