| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,485,355 | 0 | A | C | 64.3% | 4.8 / 11.6 | 14 | Y66S (TAC→TCC) | cybB | cytochrome b561 |
| Reads supporting (aligned to +/- strand): ref base A (5/0); new base C (0/9); total (5/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.00e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.61e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGT > NZ_CP009273/1485277‑1485438 | tcatcaACATGATTCATTTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTAAAATCCCCACCCccgc < 2:710139/90‑1 (MQ=255) aTTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCt > 1:513427/1‑90 (MQ=255) aTTCATGTTTCCTGTGGCATCTCAATCCCCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATCCCCACCCCCGCCGATTATACCt < 1:687407/90‑1 (MQ=255) ttCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCaaa > 2:602000/1‑90 (MQ=255) ccTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTTTTTTGTTAAGGCTGAAATCCCCACCCCCGCCGATTATACCTAAGCCAAAAc < 2:63029/90‑1 (MQ=255) cTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAAcc > 1:823945/1‑90 (MQ=255) ggCATCTCAATTCTCGTGCTGATGGTCGTTCGTTTTTTGTAAAGGCTAAAATCCCCACCCCCGCCGATTATACCTAAGCCAAAACCgatg < 1:93131/90‑1 (MQ=255) atttCTCGTGCGGATGGTCGTTCGTCTTTTGTTAAGGCTAAAATCCCCACCCCCGCCGATTATACCTAAGCCAAAACCGATGATGACggg < 1:535169/88‑1 (MQ=255) gTGCTGATGGTCGTTCGTCTTTTTTTAAGGCTGAAATCCCCACCCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGgcg < 2:172188/90‑1 (MQ=255) aTGGTCGTTCGTCTTTTGTTAAGGCTAAAATCCCCACCCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTg < 2:296097/90‑1 (MQ=255) tcgttcgtCTTTTGTTAAGGCTAAAAACCCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGAc < 1:492972/90‑1 (MQ=255) aGGCTAAAATCCCCACCCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTAtctc < 1:311874/90‑1 (MQ=255) tGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGt > 1:801260/1‑90 (MQ=255) tGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGt > 1:871509/1‑90 (MQ=255) | TCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGT > NZ_CP009273/1485277‑1485438 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |