| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,673,561 | 0 | A | C | 61.9% | 14.3 / 18.6 | 21 | Y293S (TAC→TCC) | ydgH | DUF1471 family protein YdgH |
| Reads supporting (aligned to +/- strand): ref base A (6/2); new base C (0/13); total (6/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.16e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.09e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAA > NZ_CP009273/1673489‑1673648 | gCACCTACGGCAACATGACCGAATTCTCCTATCAGGTGGCGAAACGTGCCGCGAAGAAGGGTGATAAGTATTCCCACACCCCCCGCCAGt < 1:564729/90‑1 (MQ=255) gCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTa > 2:576677/1‑64 (MQ=255) gCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTa < 1:576677/64‑1 (MQ=255) aaCATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTTTTCCCACATCCCCCGCCAGTGGCAGGAACGt < 2:686893/90‑1 (MQ=255) aCATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACggg > 1:67890/1‑88 (MQ=255) aCATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTg > 1:462117/1‑90 (MQ=255) aTGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGt > 2:755770/1‑90 (MQ=255) tGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTTTTCCCACATCCCCCGCCAGTGGCAGGAACGTGGta < 2:394019/90‑1 (MQ=255) ggTTGCGAACCGTGCCGCAAAAAAAGGTGCTAAGTATTCCCACATCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAgcgc < 2:462117/90‑1 (MQ=255) ggTTGCAAACCGGGCCGGTAAAAAAGGTAGAAAGTTTTCCCTCCCCCCCCGCCAGTGGCAGGAACGTGGTAATAGCCTGACCGTCAgcgc < 1:84173/90‑1 (MQ=255) ggTTGCAAAACGTGCCGCAAAAAAAGGTGCTAAGTATTCCCACATCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAgcgc < 2:67890/90‑1 (MQ=255) tGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGGGGTAATAACCTGACCGTCAGCGCCGa > 1:255961/1‑90 (MQ=255) caaaaCGTGCGGAGAAAAAGGGTGCAAATTTTTCCCACATCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATc < 1:221352/88‑1 (MQ=255) ccGAGAAAAAAGGTGCTAATTTTTCCCACACCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTataaat < 1:517250/90‑1 (MQ=255) aagaaAGGTGCTAATTATTACCACATCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTa < 2:495515/90‑1 (MQ=255) gTGCTAATTTTTCCCACACCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGtt < 1:542459/90‑1 (MQ=255) gTGCTAAGTTTTCCCACATCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGtt < 1:120554/90‑1 (MQ=255) gTGCTAAGTATTCCCACATCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGtt < 2:612899/90‑1 (MQ=255) gatAAGTTTTCCCACCCCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTAc < 2:617403/88‑1 (MQ=255) acaggTATTCCCCCACCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACt < 2:689092/86‑1 (MQ=255) aaGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAgcgc < 2:441660/59‑1 (MQ=255) aaGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAgcgc > 1:441660/1‑59 (MQ=255) ttACCACATCACCCGCCAGTGGCAGGAACAAGGGAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACAATTCAGGGACTTCtatta > 2:402721/1‑88 (MQ=255) | GCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAACTATTCAGTTACTCCTATAA > NZ_CP009273/1673489‑1673648 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |