| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,380,347 | 0 | T | A | 56.2% | 10.7 / 20.2 | 17 | intergenic (+37/‑66) | yfbL/yfbM | M28 family metallopeptidase/YfbM family protein |
| Reads supporting (aligned to +/- strand): ref base T (1/6); new base A (9/0); total (11/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.74e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.80e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATG‑AAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTA > NZ_CP009273/2380261‑2380429 | tGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGca < 1:390113/90‑1 (MQ=255) gTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAAAGACAATAACaatttttttt > 2:162320/1‑90 (MQ=255) gATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTccc < 1:89319/90‑1 (MQ=255) ggTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAAAGACAAGGACATTTTTTTTTTCCttc > 2:225284/1‑88 (MQ=255) gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAGAAAAACACATTTTTTTTTTCCTcct > 2:406862/1‑88 (MQ=255) cTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTaa < 1:406862/90‑1 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAAAGACAAGCACATTTTTTTTTTCCTCCTCTGtttttta > 2:376480/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAAAGACAAACAAATTTTTTTTTTTCTCCTTTGtttttaa > 1:767367/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAGACAAGCACATTTTTTTTTTCCTCATCTGTTTTTAg > 2:704626/1‑90 (MQ=255) ataCAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGa < 1:162320/90‑1 (MQ=255) aGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATG‑a < 1:283737/90‑1 (MQ=255) taataaAAATTTCTCGAGGCGGGGGCACAAAAAAAAACAAAAACAATTTTTTTTTCCCCCTCTGTTTTTATATGCGGAAAATa‑aaaataa > 1:88484/1‑86 (MQ=255) ataaAAATTTCTCGAGGCGGGGCCAAAAAAAACGACACGCACATTTTTTTTTTCCTCCTCTTTTTTAAAATGCGAAAAAAG‑AAAATCAAc > 2:623340/1‑90 (MQ=255) aaaaTTTCTCGAGGCGGGGCCAGAAAAAAAGACAAGCACATTTTTTTTTTCCTCATCTGTTTTTAGATGGGAAAAAAGAAAATTAAACgg > 2:180195/1‑90 (MQ=255) aGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATG‑AAATTCAACGGAGGAAaaataaa < 1:268184/90‑1 (MQ=255) gCGGGGCCAGAAAAAAAGACAAGCCCATTTTTTTTTTTCTCCTCTGTTTTTTGATGGGAAAAAAG‑AAAATCAACGGAGGAaaaaaaaaag > 1:235391/1‑88 (MQ=255) aGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATG‑AAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTa > 2:269533/1‑90 (MQ=255) | TGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATG‑AAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTA > NZ_CP009273/2380261‑2380429 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |