| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,738,448 | 0 | A | C | 62.5% | ‑0.1 / 10.9 | 16 | V84G (GTG→GGG) | trmD | tRNA (guanosine(37)‑N1)‑methyltransferase TrmD |
| Reads supporting (aligned to +/- strand): ref base A (3/3); new base C (0/10); total (3/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.57e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.47e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTCCGCC > NZ_CP009273/2738359‑2738533 | gACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCa > 2:624057/1‑90 (MQ=255) cacCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTgc < 1:91314/90‑1 (MQ=255) cacCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTgc < 1:293774/90‑1 (MQ=255) ttGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCCCCcgccgc < 1:673442/90‑1 (MQ=255) gATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCgg > 1:692890/1‑90 (MQ=255) gCCATTTGGGTGCCGCCGGCTTGCTAAAGTTTGCTCCCGCTTGGTGACAGATAAATCCCTTTGGCGCTTTCCCCCGCCGCGGCTTTtgct < 1:861108/90‑1 (MQ=255) gCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTtgct < 2:379765/90‑1 (MQ=255) cGCCCGCTTGATCAAGCTTGCGCCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGt < 2:190637/90‑1 (MQ=255) ccccGCTTGACCAAGCTTGCGCCCCTGTGGTGAAAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTc < 1:503797/89‑1 (MQ=255) tCAAGCTGGCGCCCCTGTGGTGCAAGATAAATCCCTTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGt < 1:688053/90‑1 (MQ=255) aGCTTGCGTCCTTGTGGTGACAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGc < 1:400544/90‑1 (MQ=255) aGCTTGCGTCCTTGTGGTGAAAGAAAAATCCCCTTTGCCCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGc < 1:778960/90‑1 (MQ=255) cTTGCGTCCCTGTGGTGACAAAAAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAc < 1:35867/90‑1 (MQ=255) cTTGCGTCCCTGCGGTAAAAGAAAAATCCCCTTTGCGCCTCCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAc < 1:46884/90‑1 (MQ=255) ccTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTa > 1:319227/1‑90 (MQ=255) aTAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTcc > 1:799596/1‑90 (MQ=255) aaTCCCCTTTGCCCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTccgcc < 2:441729/90‑1 (MQ=255) | GACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTCCGCC > NZ_CP009273/2738359‑2738533 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |