| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,225,954 | 0 | A | G | 58.8% | 4.1 / 16.0 | 17 | S41G (AGT→GGT) | yjbE | exopolysaccharide production protein YjbE |
| Reads supporting (aligned to +/- strand): ref base A (2/5); new base G (10/0); total (12/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.39e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGCGGTGATGGTTCTAATACC > NZ_CP009273/4225876‑4226040 | gcggcgACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGGGGTCCTCcccg > 2:549936/1‑90 (MQ=255)gcggcgACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGGGGTCCTCcccg > 2:193098/1‑90 (MQ=255) gCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGc < 1:396399/90‑1 (MQ=255) gTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGGGGTCCTCCCCGGCCAGCGGCAGCCCCCTAAGGTcgggg > 2:406812/1‑88 (MQ=255) tACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGGGGTCCTCCCCGACCCGCGGCAGCACCCTAAGCTcggcgg > 2:739048/1‑90 (MQ=255) aGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGGGGTCCTCCCCGACCAGCGGCAGCACCGTAAGCTCCGCggggg > 2:160493/1‑90 (MQ=255) tGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGGGGTTCTCCCCCACCAGCGGCAGCACCGTAAACTCCgggggggggg > 2:250201/1‑88 (MQ=255) tGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGGGGTCCTCCCCGACCAGCGTCAGCACCGGAAGATCGGCgggggggg > 2:377967/1‑88 (MQ=255) ggCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGGGGTCCTCCCCGACCAGCGGCAGCACCGTAAGCTCGGCgggggggggg > 2:825343/1‑86 (MQ=255) ggCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGGGGTCCTCCCCGACCAGCGGCAGCACCCTAAGCTCGGCgggggggggt > 2:571927/1‑86 (MQ=255) cGGCAGCAACGTCGGTTTCGGCGGGGGGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGGGGGGGGCGCGCTTGCGGCAACCg > 2:406242/1‑90 (MQ=255) aCGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGGGGGGGTCGCGCTTGCGGGAACCCGTGggggg > 2:103066/1‑89 (MQ=255) gTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGCGGTGa < 1:549936/90‑1 (MQ=255) cGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGCGGTGATg < 1:862634/90‑1 (MQ=255) cGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGCGGTGATg < 1:185419/90‑1 (MQ=255) ggggAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGCGGTGATGGTTCTAATAc < 1:103066/90‑1 (MQ=255) gggAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGGGGGGGGCGCGCTTGCGGCAACCCGGGGCGGGGGTGGTTTTAATAcc > 1:780408/1‑90 (MQ=255) | GCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGCGGTGATGGTTCTAATACC > NZ_CP009273/4225876‑4226040 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |