| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,245,973 | 0 | T | G | 62.5% | 9.5 / 11.7 | 16 | N141T (AAC→ACC) | plsB | glycerol‑3‑phosphate 1‑O‑acyltransferase PlsB |
| Reads supporting (aligned to +/- strand): ref base T (1/5); new base G (10/0); total (11/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.37e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.30e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGC > NZ_CP009273/4245896‑4246062 | aaCGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCtt < 1:383251/90‑1 (MQ=255) caAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCCTTTTTAc > 2:329653/1‑90 (MQ=255) cTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGcccc < 1:708050/90‑1 (MQ=255) gTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTGCCGTCAGCTTTTTTTCGcccccg > 1:452907/1‑88 (MQ=255) gACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCCCTTCGCCCTTTTTACGCCCCcgcgcgg > 2:81172/1‑89 (MQ=255) cAGCG‑AAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGa < 2:728418/90‑1 (MQ=255) aGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTGACTTTCCCGTTTTTATGCCCCCGCGCGCGGGCAAACAACAccag > 2:261975/1‑88 (MQ=255) gCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCTTTTTTACGCCCCCGCGCGCGGCCAAAAATCACCggc > 2:697733/1‑88 (MQ=255) aaaTTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCTTTTTTTCCCCCCCGCGCGCCGCCCAACAACCCCCACACCgg > 1:850028/1‑90 (MQ=255) aaaTTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCTTTTTTACGCCCCGGCGCGCGACCAAAAATCACCCACACTgg > 2:296077/1‑90 (MQ=255) aaaTTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCTTTTTTACCCCCCGGCCCGCGCCCAAACATCACCCACACTgg > 1:698742/1‑90 (MQ=255) aaaTTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCTTTTTTACCCCCCCGCGCGCGACCAAACATCACCCACACCgg > 2:576313/1‑90 (MQ=255) aaaTTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACCTCTCCCTTTTTTCCCCCCCGCGCGCCACCAAACACCACCGCCACCgg > 1:129803/1‑90 (MQ=255) tttCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCAc < 1:107229/90‑1 (MQ=255) gTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTTACGCCCCCGCGCGCGACCAAAAATCACCCACACTGGCACCATCTGCACATc > 2:739820/1‑90 (MQ=255) aTACGCAGCGGCGGGTTCACTTCGCCTTTTTCCCGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGAttt < 1:329653/90‑1 (MQ=255) ttCACTTCGCCTTTTTCACGCCCCGGCGCGCGCCCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGc < 2:134066/90‑1 (MQ=255) ttCACTTCGCCTTTTTCACGCCCCAGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGc < 1:222905/90‑1 (MQ=255) | AACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGC > NZ_CP009273/4245896‑4246062 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |