| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,290,979 | 0 | T | G | 57.1% | 2.7 / 12.8 | 14 | T639T (ACA→ACC) | mdtO | multidrug efflux transporter permease subunit MdtO |
| Reads supporting (aligned to +/- strand): ref base T (1/5); new base G (8/0); total (9/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.00e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGCGTCGGCAAAC > NZ_CP009273/4290891‑4291060 | gggCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGgtg < 2:498133/90‑1 (MQ=255) gCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGtc < 1:365495/90‑1 (MQ=255) tCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGGCTCtt > 2:25521/1‑90 (MQ=255) gCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGGGTCTCTTCAAGCggtt > 2:284955/1‑87 (MQ=255) gCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGGGGCTCTTTAAGCGTTTTTTg > 2:449585/1‑90 (MQ=255) cGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTga < 1:21552/90‑1 (MQ=255) cGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGGGGCTCTTCAAGCGGTAtttta > 2:78133/1‑88 (MQ=255) ggCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTgagg < 2:39526/90‑1 (MQ=255) ggCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGGGGCTCTTCTAGGGGTAtttttgg > 2:361577/1‑86 (MQ=255) tGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGGGTCTCTTCAAGCGTTATTTTAGGGGAACGGCTg > 2:833469/1‑90 (MQ=255) gtgtTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGt < 1:266782/90‑1 (MQ=255) gTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGGGGCTCTTTAAGCGTTATTTTAGGGGAACGGCTGGGGGCGGGTGCCAGACCgg > 2:797810/1‑90 (MQ=255) gTGGGCAGGATGGCCTGAGAGGCGGGGGTCTCTTCAAGCGTTATTTTAGGGGAACGGCTGAGGGGGGGTGGCCGACCGGCAGGGGAtttt > 2:856760/1‑89 (MQ=255) tGGGCAGGATGGCCTGAGAGGCGGGGGTCTCTTTTAAAGTTATTTGGGGGGAAAGGGTGGGGGGGGGTGGCAGACCCGCAGCGGAttttt > 1:271559/1‑88 (MQ=255) gagGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGCGTCGGCAAAc < 1:391675/90‑1 (MQ=255) | GGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGCGTCGGCAAAC > NZ_CP009273/4290891‑4291060 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |