Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,370,599	T→G	T61P (ACC→CCC)	sspA ←	stringent starvation protein A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,370,599	0	T	G	100.0%	120.0 / NA	32	T61P (ACC→CCC)	sspA	stringent starvation protein A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (17/15);  total (17/15)

CAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATC‑‑CACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACC  >  NZ_CP009273/3370516‑3370683                                                                                      |                                                                                     cAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATC‑‑CACCAGGGGCGGAAc                                                                                <  2:282867/90‑1 (MQ=255)     ggCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATC‑‑CACCAGGGGCGGAACGctc                                                                            <  1:4635/90‑1 (MQ=255)     ggCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATC‑‑CACCAGGGGCGGAACGctc                                                                            <  1:711059/90‑1 (MQ=255)      gCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATC‑‑CACCAGGGGCGGAACGctct                                                                           >  2:518679/1‑90 (MQ=255)      gCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGGCAGCTCACGATC‑‑CACCAGGGGCGGAACGctct                                                                           >  1:363901/1‑90 (MQ=255)                    cGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATC‑‑CACCAGGGGCGGAACGCTCTGATTCGGGTTGAgg                                                             <  1:852163/90‑1 (MQ=255)                        cATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTc                                                           <  1:57992/90‑1 (MQ=255)                            cAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATC‑‑CACCAGGGGCAGAACGCTCTGATTCGGGTTGAGGTCAATCAg                                                     <  1:165024/90‑1 (MQ=255)                                    ccATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATC‑‑CACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAg                                             >  1:293863/1‑90 (MQ=255)                                    ccATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATC‑‑CACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAg                                             >  2:492565/1‑90 (MQ=255)                                    ccATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATC‑‑CACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAg                                             >  2:403774/1‑90 (MQ=255)                                    ccATAATGATGCGAGATTCCCACAGGGTCAGATCACGATC‑‑CACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAg                                             >  2:436390/1‑90 (MQ=255)                                     cATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATC‑‑CACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAgg                                            <  1:518679/90‑1 (MQ=255)                                     cATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATC‑‑CACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAgg                                            <  1:97104/90‑1 (MQ=255)                                        aaTGATGCGAGATTCCCACAGGGTCAGCTCACGATC‑‑CACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCgg                                         >  2:648892/1‑90 (MQ=255)                                         atgatgCGAGATTCCCACAGGGTCAGCTCACGATC‑‑CACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGa                                        >  2:703505/1‑90 (MQ=255)                                           gatgCGAGATTCCCACAGGGTCAGCTCACGATC‑‑CACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGAtt                                      >  1:275721/1‑90 (MQ=255)                                            atgCGAGATTCCCACAGGGTCAGCTCACGATC‑‑CACCAGGGGCGGAACGctc                                                                            <  1:459033/51‑1 (MQ=255)                                            atgCGAGATTCCCACAGGGTCAGCTCACGATC‑‑CACCAGGGGCGGAACGctc                                                                            >  2:459033/1‑51 (MQ=255)                                            atgCGAGATTCCCACAGGGTCAGCTCACGATC‑‑CACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTg                                     >  1:382113/1‑90 (MQ=255)                                            atgCGAGATTCCCACAGGGTCAGCTCACGATC‑‑CACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTg                                     >  2:525637/1‑90 (MQ=255)                                             tgCGAGATTCCCACAGGGTCAGCTCACGATC‑‑CACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGt                                    >  2:235942/1‑90 (MQ=255)                                                             ggTCAGCTCACGATC‑‑CACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCa                           <  1:509251/83‑1 (MQ=255)                                                             ggTCAGCTCACGATC‑‑CACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCa                           >  2:509251/1‑83 (MQ=255)                                                                cAGCTCACGATC‑‑CACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGAt                 >  2:361559/1‑90 (MQ=255)                                                                cAGCTCACGATC‑‑CACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGAt                 >  1:297196/1‑90 (MQ=255)                                                                cAGCTCACGATC‑‑CACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGAt                 >  2:574801/1‑90 (MQ=255)                                                                     cACGATC‑‑CACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGa            <  1:648892/90‑1 (MQ=255)                                                                     cACGATC‑‑CACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGa            <  1:266661/90‑1 (MQ=255)                                                                                 caGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACAcc  <  1:703505/90‑1 (MQ=255)                                                                                 caGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACAcc  <  2:206605/90‑1 (MQ=255)                                                                                 caGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACAcc  <  1:228197/90‑1 (MQ=255)                                                                                      |                                                                                     CAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATC‑‑CACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACC  >  NZ_CP009273/3370516‑3370683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑