| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 226,010 | (A)6→5 | coding (357/804 nt) | dkgB → | 2,5‑didehydrogluconate reductase DkgB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 226,005 | 0 | A | . | 94.4% | 65.7 / ‑1.3 | 18 | coding (352/804 nt) | dkgB | 2,5‑didehydrogluconate reductase DkgB |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base . (8/9); total (9/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATCTGACGCTAATCCACTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTGACGCGTGAGATCGGTATTTCCAACTTCACGATCCCGTTGATGGAAAAAGCGATTGC > NZ_CP009273/225927‑226075 | gATCTGACGCTAATCCACTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAggg > 1:547279/1‑90 (MQ=255) tCTGACGCTAATCCACTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCC‑AAAAACAAGGGCTg > 1:67767/1‑90 (MQ=255) gCTAATCCACTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCC‑AAAAACAAGGGCTGACGCGt < 1:79443/90‑1 (MQ=255) ccACTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCC‑AAAAACAAGGGCTGACGCGTGAGATc < 1:323767/90‑1 (MQ=255) tGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCC‑AAAAACAAGGGCTGACGCGTGAGATCGGTa < 2:37879/90‑1 (MQ=255) tGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCC‑AAAAACAAGGGCTGACGCGTGAGATCGGTa < 2:1660/90‑1 (MQ=255) ggCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCC‑AAAAACAAGGGCTGACGCGTGAGATCGGTAt > 1:208412/1‑90 (MQ=255) ggCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCC‑AAAAACAAGGGCTGACGCGTGAGATCGGTAt > 1:208413/1‑90 (MQ=255) ggCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCC‑AAAAACAAGGGCTGACGCGTGAGATCGGTAt > 1:386092/1‑90 (MQ=255) aCGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCC‑AAAAACAAGGGCTGACGCGTGAGATCGGTATTTCCAACTTCAc < 1:447228/90‑1 (MQ=255) aTGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCaaaaaca < 1:259509/51‑3 (MQ=255) aTGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCaaaaaca > 2:259509/1‑49 (MQ=255) tGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCC‑AAAAACAAGGGCTGACGCGTGAGATCGGTATTTCCAACTTCACGATc > 2:535749/1‑90 (MQ=255) ctGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCC‑AAAAACAAGGGCTGACGCGTGAGATCGGTATTTCCAACTTCAc > 1:474405/1‑78 (MQ=255) ctGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCC‑AAAAACAAGGGCTGACGCGTGAGATCGGTATTTCCAACTTCAc < 2:474405/78‑1 (MQ=255) ctGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCC‑AAAAACAAGGGCTGACGCGTGAGATCGGTATTTCCAACTTCACGATCCCGTTGAt < 1:477870/90‑1 (MQ=255) gTTTATGCAGGCGCTGCTGGAAGCC‑AAAAACAAGGGCTGACGCGt > 1:463692/1‑45 (MQ=38) gTTTATGCAGGCGCTGCTGGAAGCC‑AAAAACAAGGGCTGACGCGt < 2:463692/45‑1 (MQ=38) gTTTATGCAGGCGCTGCTGGAAGCC‑AAAAACAAGGGCTGACGCGTGAGATCGGTATTTCCAACTTCACGATCCCGTTGATGGAAAAAGCg > 1:189273/1‑90 (MQ=255) tGCAGGCGCTGCTGGAAGCC‑AAAAACAAGGGCTGACGCGTGAGATCGGTATTTCCAACTTCACGATCCCGTTGATGGAAAAAGCGATtgc < 1:395509/90‑1 (MQ=255) | GATCTGACGCTAATCCACTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTGACGCGTGAGATCGGTATTTCCAACTTCACGATCCCGTTGATGGAAAAAGCGATTGC > NZ_CP009273/225927‑226075 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |