| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,329,948 | 0 | T | G | 53.8% | 12.6 / 17.1 | 13 | intergenic (+24/‑140) | BW25113_RS25560/acnA | hypothetical protein/aconitate hydratase AcnA |
| Reads supporting (aligned to +/- strand): ref base T (1/5); new base G (7/0); total (8/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTA > NZ_CP009273/1329864‑1330033 | gggCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCa > 1:65808/1‑90 (MQ=255) gcgcGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCAtt < 2:65808/90‑1 (MQ=255) gTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCATTTGCTTTGCCACaa > 2:484069/1‑90 (MQ=255) aTTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCATTTTCTTTTCCACAAGGTTTTTCCTCTTTTTTCAATTTTGGTTGTTTTc > 1:237128/1‑90 (MQ=255) aTTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCATTTTCTTTTCCACAAGGTTTTTCCTCTTTTTTCAATTTTGGGTGTTATc > 1:24046/1‑90 (MQ=255) tGATCCTTGACCCCGCTGCGGCGGGGGTGTTCTTTGCTTTTCCACAAAGTTTTTCCTCTTTTTTCAATTTGGGGTTTTTTTAAAACGTTa > 1:295371/1‑90 (MQ=255) gATCCTTGACCCCGCTGCGGCGGGGGTGTCATTTTCTTTTCCACAAGGTTTTTCCTCTTTTTTTAATTTGGGGTGTTTTCAAATCGtttc > 1:486376/1‑88 (MQ=255) aCCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGtt > 2:408491/1‑90 (MQ=255) aCCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGtt < 2:424159/90‑1 (MQ=255) aCCCCGCTGCGGCGGGGGTGTCATTTTCTTTGCCACAAGGTTTTTCCTCTTTTTTTAATTTGGGGTTTTTTCAAATCGTTACGCGATGtt > 1:495378/1‑90 (MQ=255) gCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTgtgt < 1:408491/90‑1 (MQ=255) tGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTa < 2:425256/90‑1 (MQ=255) ggggTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTa < 2:55203/90‑1 (MQ=255) | GGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTA > NZ_CP009273/1329864‑1330033 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |