| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,089,571 | 0 | A | G | 54.5% | 4.6 / 11.9 | 11 | I83V (ATT→GTT) | hisF | imidazole glycerol phosphate synthase subunit HisF |
| Reads supporting (aligned to +/- strand): ref base A (0/5); new base G (6/0); total (6/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.16e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCA > NZ_CP009273/2089485‑2089648 | cTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTa < 2:195162/90‑1 (MQ=255) cAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGtc < 2:373009/90‑1 (MQ=255) gCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTGGAAGATGCCCCGAAAAATtttt > 2:276872/1‑90 (MQ=255) gggTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTGGAAGATGCCCCGAAAAATTTTTTCt > 1:293209/1‑90 (MQ=255) cgTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTa > 1:440105/1‑47 (MQ=255) cgTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTa < 2:440105/47‑1 (MQ=255) gATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTTAGTCTCTGGGAGAAGCCCCCAAAATTTTTTTCTTTGGGGGGGGGAAAAATTTCAt > 1:66454/1‑90 (MQ=255) aCATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTTGCGCGGGTAAAAATTCCATCAAct > 2:56328/1‑90 (MQ=255) tttGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTgcgc < 2:132360/90‑1 (MQ=255) ttGTGTGGCGGGGGGGGTTTAGTCTCTGGAAGATGCCGCGAAAAATTTTTCCTTTTGGGCGGAGAAAAATTCCATCAACTCTCCTgcgcc > 2:274768/1‑89 (MQ=255) gtgGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCa < 2:158051/90‑1 (MQ=255) | CTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCA > NZ_CP009273/2089485‑2089648 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |