| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,212,359 | 0 | A | C | 54.5% | 8.1 / 11.5 | 11 | V201G (GTG→GGG) | osmF | glycine betaine ABC transporter substrate‑binding protein OsmF |
| Reads supporting (aligned to +/- strand): ref base A (4/1); new base C (0/6); total (4/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.52e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TAAGCCATTGCAGCATTAACGCCAGAGGTTTGCTGGGCAGCGGCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCTTTTTCAAACGCGGGT > NZ_CP009273/2212305‑2212436 | tAAGCCATTGCAGCATTAACGCCAGAGGTTTGCTGGGCAGCGGCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGa > 2:276807/1‑90 (MQ=255) aGCCATTGCAGCATTACCGCCAGAGGTTTGCTGGGCAGCGGCTTTGACCGTCCCCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATc < 1:9826/90‑1 (MQ=255) aTTGCAGCATTAACGCCAGAGGTTTGCTGGGCAGCGGCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGa > 2:349883/1‑90 (MQ=255) ttGCAGCATTAACGCCAGAGGTTTGCTGGGCAGCGGCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGAc > 2:191780/1‑90 (MQ=255) gcagcaTTACCGCCAGAGGTTTGCGGGGCAGCGGTTTTGACCGTCCCCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCg < 1:437960/90‑1 (MQ=255) gcagcaTTAACGCCAGAGGTTTGCTGGGCAGGGGTTTTGACCGTCCCCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCg < 1:492071/90‑1 (MQ=255) taccGCCAGAGGTTTGCGGGGAAGGGGTTTTGATCGTCCCCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTaa < 1:533123/87‑1 (MQ=255) tAACGCCAGAGGTTTGCTGGGCAGCGGCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTaa < 2:95915/90‑1 (MQ=255) ttGCTGGGCAGCGGCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCttt > 1:219136/1‑90 (MQ=255) ggCGGTTTTGATGGTCCCCGCCGTTCCGCCCCCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCTTTTTCAAAcgc < 1:349883/89‑1 (MQ=255) gttttGATCGTCCCCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCTTTTTCAAACGCGGGt < 1:212537/88‑1 (MQ=255) | TAAGCCATTGCAGCATTAACGCCAGAGGTTTGCTGGGCAGCGGCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCTTTTTCAAACGCGGGT > NZ_CP009273/2212305‑2212436 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |