| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,436,636 | 0 | A | C | 68.8% | ‑1.8 / 11.2 | 16 | V274G (GTA→GGA) | secY | preprotein translocase subunit SecY |
| Reads supporting (aligned to +/- strand): ref base A (5/0); new base C (0/11); total (5/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.29e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.23e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGT > NZ_CP009273/3436558‑3436714 | cAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCata > 2:66503/1‑90 (MQ=255) cccccccAACCCAGGGCGCGTTGGGCGCCGGAAAAAAAATAATACTGGAAGGAAAGATGGCGGGAATACCCCCCGCCATATTCACTTTCa < 2:379252/88‑1 (MQ=255) cccccccAACCCAGGCCGCGAGGGCCGCCGGAAAAAAAAAAATCCGGGAAGAAAAGTTTGCCGGGATCCCCCCCGCCATATTCACTTTCa < 1:80747/88‑1 (MQ=255) gCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAg > 2:35216/1‑90 (MQ=255) cccccGACCCAGGACGGGGTGGCCGCGGGAAAAAAAATAATACTGGAAGCGAAGATGGCGGGGTTTCCCCCCGCCATATTCACTTTCAGc < 2:350107/90‑1 (MQ=255) gAACCATGACGCGAGGGTCGCCGGAAAAAAAATAATACTGGAAGCGAAGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTaa < 2:195893/90‑1 (MQ=255) aTGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAAtgtg > 2:206792/1‑90 (MQ=255) ggggtGGCCGCCGGAAAAAAAATAATACTGAAAGAAAAGTTGGCCGGATTCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGctc < 1:99518/86‑1 (MQ=255) gggATGGTCCCCGGAAAAAAAATAATCCGGGAAGAGAAGTTTGCCGGTTTCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGctc < 1:161210/88‑1 (MQ=255) gggATGGCCGCGGGAAAAAAAAAAATACTGAAAGGAAAGTTTGCGGGAATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGttc < 2:299072/88‑1 (MQ=255) gcgcTGGTCGCCGGGAAGAAAAAAATACTGGGAGAAAAGGTTGCGGGCATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGctc < 1:459894/90‑1 (MQ=255) tGGCCGCGGGAAACAAAATAATACTGGAAGAGAAGTTTGCCGGTATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCtgtg < 2:211270/90‑1 (MQ=255) cGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgcagc > 2:459894/1‑90 (MQ=255) aaGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGAc > 1:536114/1‑90 (MQ=255) ttGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGt < 2:78358/90‑1 (MQ=255) tggCCGGAATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGt < 2:300359/88‑1 (MQ=255) | CAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGT > NZ_CP009273/3436558‑3436714 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |