| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,571,657 | 0 | G | A | 54.5% | 2.5 / 10.8 | 11 | P144S (CCC→TCC) | gntR | gluconate operon transcriptional repressor GntR |
| Reads supporting (aligned to +/- strand): ref base G (4/1); new base A (0/6); total (4/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.52e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAACCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCAT > NZ_CP009273/3571568‑3571744 | aGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAACCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACggg > 2:177042/1‑90 (MQ=255) cctTAAAATTTAAAAAACCCGACGGCAATACAAAGGCAGGGCGACTTGCTGCCCATCAGTCCCCCCACGGAAATCCCCGCCACTTCAatc < 2:527613/89‑1 (MQ=255) ttCAAAGTTATCAAAACCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAatcat > 2:369123/1‑90 (MQ=255) tATCAAAACCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAgg > 1:235515/1‑90 (MQ=255) cccGACGGCGATATCAAGGCATGGCGTCTTGCTGTCCATCAGTCCCCCCAGGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCgcgg < 2:76256/89‑1 (MQ=255) gCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTgg > 1:76256/1‑90 (MQ=255) agggCAGGGCGTTTTGCTGCCCATCATTCCCCCCGGGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTc < 1:52247/88‑1 (MQ=255) cagggCGCCTTGCTCCCCATCATTCCCCCCACGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTg < 2:205009/87‑1 (MQ=255) aTGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGa > 1:205009/1‑90 (MQ=255) gaTCTGTCCCCCCACGGGAACCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGat < 1:253289/89‑1 (MQ=255) cATCAGTCCCCCCGGGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCACGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGat < 1:177042/90‑1 (MQ=255) cATCAGTCCCACCAGGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGat < 2:235515/90‑1 (MQ=255) gggAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCAt > 1:127249/1‑90 (MQ=255) | AGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAACCGACGGCGATATCAAGGCATGGCGACTTGCTGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCAT > NZ_CP009273/3571568‑3571744 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |