| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,806,632 | 0 | C | T | 48.3% | 10.9 / 19.8 | 29 | S181L (TCG→TTG) | coaBC | bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate‑‑cysteine ligase CoaBC |
| Reads supporting (aligned to +/- strand): ref base C (3/12); new base T (14/0); total (17/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.05e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCGGTGCGTTATATCTCTA > NZ_CP009273/3806553‑3806718 | aGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAAc < 2:89795/90‑1 (MQ=255) gTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCCTTTTTTTCCCCTCCACg > 2:398184/1‑90 (MQ=255) ttGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGaa < 1:67281/90‑1 (MQ=255) gtggtgATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAAc < 2:234334/90‑1 (MQ=255) gATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGcc < 1:64927/68‑1 (MQ=255) gATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGcc > 2:64927/1‑68 (MQ=255) gATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGGAGGGCGTTTTTTTCCCCTCCACGACCTGAAAAAACTg > 1:408746/1‑90 (MQ=255) gATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGGAGGGCGTTTTTTTCCCCCCCACGACCCGAAACATCTg > 1:235888/1‑90 (MQ=255) ggTCCTGGGCGAATGCCCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTTTCCCCCCCACGACCCGAAAAAACTTAACAtt > 1:181313/1‑90 (MQ=255) gggCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGAtt < 2:408746/90‑1 (MQ=255) aaTGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCCTTTTTTTCCCGCCCACGACCTGTAAAATCTTAACATTATGATTAccgc > 1:349731/1‑90 (MQ=255) aaTGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCCTTTTTTTCCCCCCAACGACCCGAAAAATCTGAACATTATGATTAcccc > 1:435057/1‑88 (MQ=255) aaTGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAAAATCTGAACATTATGATTAccgc > 2:179676/1‑90 (MQ=255) tCGATCCGTTAACCATTGTGGATATGGCGGGAGGGCATTTTTTTCCCCCCCACCACCCGAAAAAATTGAAAATTATTATTACCCCCCGcc > 1:474603/1‑90 (MQ=255) gTTAACCATTGTGGATATGGCGGTAACGCATTTTTTGCCCGCCAACGACCTGAAAAAACTGAACATTATGATTACCCCCCGCCCGAcccg > 2:352600/1‑90 (MQ=255) ttGTGGATATGGCGGTAGTGCATTTTTTGCCCCTCCACGACCTGAAAAATCTGAAAATTATGATTACCCCCCGCCCCGCGCGGGAACCGc > 1:120665/1‑90 (MQ=255) ttGTGGATATGGCGGTAGCGCATTTTTTGCCCGTCAACg > 1:179859/1‑39 (MQ=255) ttGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACg < 2:179859/39‑1 (MQ=255) ttGTGGATATGGCGGGAGCGCATTTTTTTCCCGTCAACGACCCGAAAAATCTGAAAATTATGATTACCGCCGGCCCCACCCGGGAACCGc > 1:63872/1‑90 (MQ=255) gtgGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTc < 1:227155/90‑1 (MQ=255) tGGCGGTAGCGCATTTTTTGCCCGTCAACGACCTGAAAAATCTGAACATTATGATTACCCCCGGCCCGACGCGTGAAACGCTCGATCCgg > 2:227155/1‑90 (MQ=255) tGGCGGGAGCGCATTTTTTGCCCCTCAACGACCTGAAACAACTGAAAATTATTATTACCGCCCGCCCCACGCGGGGACCGCCCCATCCgg > 1:144295/1‑90 (MQ=255) gCGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCGGTg < 2:106087/90‑1 (MQ=255) cGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCGGTGc < 2:23324/90‑1 (MQ=255) gTAGCGCATTTTTTGCCCGTCAACGACCTGAAAAATCTGAACATTATGATTACCCCCGGCCCGACGCGGGAACCGCCCGATCCGGGGCGt > 1:74334/1‑90 (MQ=255) gtgcATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCGGTGCGTtat < 2:120665/88‑1 (MQ=255) gcgcATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCGGTGCGTtat < 2:63872/90‑1 (MQ=255) gcATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCGGTGCGTTatat > 2:488332/1‑90 (MQ=255) tttCGCCCGTCAACGCCCTGAAACATCTGAACATTATGATTCCCGCCGGCCCGACGCGTGAACCGCTCGATCCGGTGCGTTATATCTCTa < 1:99328/90‑1 (MQ=255) | AGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCGGTGCGTTATATCTCTA > NZ_CP009273/3806553‑3806718 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |