| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,544,923 | 0 | C | A | 52.9% | 9.2 / 25.2 | 17 | N177K (AAC→AAA) | uxuR | Uxu operon transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (1/7); new base A (9/0); total (10/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.11e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.53e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCG > NZ_CP009273/4544842‑4544990 | gTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGGAAAAAAACCAAt > 2:78235/1‑90 (MQ=255) ttCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAAtg < 1:106802/90‑1 (MQ=255) tCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGGAAAAAAACCAAagt > 2:106802/1‑90 (MQ=255) aTCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAAACCAATTTggg > 2:470006/1‑89 (MQ=255) aGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGGGAAAAAACCAATTAGGGGCCAGGTTCCCAGCCa > 1:458655/1‑90 (MQ=255) agaCGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATc < 2:198042/88‑1 (MQ=255) gCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGGAAAAAAAACAAAGTGGGGCCCGTTTTACAGCCCTCTTGGTGACaact > 1:198042/1‑87 (MQ=255) tGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAAAAAACCAAAGTGGATCCCGTTGCACAACCCTCTGGATGACAGCCCGt > 2:40892/1‑90 (MQ=255) ggAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCaa < 1:377382/90‑1 (MQ=255) aGCTGTTCCGTCAGTCCTGGCAGTGGCGGGGAAAAAAACCAATGTGGGTCCAGTTGCACAGCCCTCCGGGTGGCAGCCTGTATCGCaaaa > 1:470276/1‑89 (MQ=255) ttCCGTCAGTCCTGGCAGTGGCGGGGAAAAAAACCAAAGTGGGTCCCGTTGCACAGCCCTCTGGGTGACAGCCCGTATCGCCAAGAgggg > 1:185444/1‑90 (MQ=255) gTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGcc > 2:221554/1‑69 (MQ=255) gTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGcc < 1:221554/69‑1 (MQ=255) cAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGc < 2:185444/90‑1 (MQ=255) cAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGc < 1:470006/90‑1 (MQ=255) cAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGc < 1:40892/90‑1 (MQ=255) aGTCCTGGCAGTGGCGGGAAAAAAAAACAAAGTGGATCCCGTTGCCCAGCCATCTGGGTGACAACCCGTATTGCAAAGAGGGGGTggggg > 1:321/1‑88 (MQ=255) | GTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGGAAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCG > NZ_CP009273/4544842‑4544990 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |